Retroviral Expression Cloning Using an Arrayed Full Length cDNA Gene Set

使用阵列全长 cDNA 基因集进行逆转录病毒表达克隆

• 批准号: nhmrc : 401608
• 负责人: Prof Thomas Gonda
• 金额: $ 122.79万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Strategic Awards
• 财政年份: 2006
• 资助国家: 澳大利亚
• 起止时间: 2006-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/retroviral-expression-cloning-gene-set/82437
• 关键词: Retroviral; Expression; Cloning; Using; Arrayed

The sequencing of the human genome has revealed the blueprint for life, but the identities and-or functions of the majority of genes remain unknown. Here we propose to establish a radically modified retroviral expression cloning system that will, in principle, allow identification of all genes that confer a particular dominant phenotype. To do this we will establish an arrayed retroviral library of sequence-verified genes covering the entire human transcriptome. This technology will be used to identify genes involved in a wide range of medically-important biological processes.

人类基因组测序揭示了生命的蓝图，但大多数基因的身份和功能仍然未知。在这里，我们建议建立一个从根本上修改的逆转录病毒表达克隆系统，将在原则上，允许识别的所有基因，赋予一个特定的显性表型。为此，我们将建立一个覆盖整个人类转录组的序列验证基因阵列逆转录病毒文库。这项技术将用于识别参与广泛的医学重要生物过程的基因。