Analysis of intracellular signalling and biological activities of the GM-CSF receptor family using constitutive mutants

使用组成型突变体分析 GM-CSF 受体家族的细胞内信号传导和生物活性

• 批准号: nhmrc : 104877
• 负责人: Prof Thomas Gonda
• 金额: $ 33.72万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2000
• 资助国家: 澳大利亚
• 起止时间: 2000-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/analysis-intracellular-signalling-constitutive-mutants/98983
• 关键词: Analysis; intracellular; signalling; biological; activities

The cytokines GM-CSF, IL-3 and IL-5 stimulate the growth and actions of a wide range of blood cells. Each binds to a receptor on the cell surface which then triggers the generation of a number of signals inside the cell; it is these signals that are responsible for the cytokine?s actions. We have previously generated a panel of constitutive mutant forms of the beta subunit which is shared by the GM-CSF, IL-3 and IL-5 receptors. These constitutive mutants trigger signals even in the absence of the cytokine; importantly, the different mutants appear to trigger only a subset of the signals generated by the normal receptor. The aim of this project is to use our panel of constitutive beta subunit mutants to determine how the GM-CSF, IL-3 and IL-5 receptors generate signals inside the cell and how these signals lead to the various biological actions of the receptors on blood cell growth and maturation. Findings from this research will be relevant to the understanding and treatment of diseases which involve abnormal growth or function of blood cells such as leukaemia and inflammatory diseases.

细胞因子GM-CSF、IL-3和IL-5刺激多种血细胞的生长和活动。每一种都与细胞表面的受体结合，然后触发细胞内产生许多信号;正是这些信号负责细胞因子？的行动。我们先前已经产生了一组由GM-CSF、IL-3和IL-5受体共享的β亚基的组成性突变形式。这些组成型突变体即使在细胞因子不存在的情况下也会触发信号;重要的是，不同的突变体似乎仅触发由正常受体产生的信号的子集。该项目的目的是使用我们的组成性β亚基突变体组来确定GM-CSF、IL-3和IL-5受体如何在细胞内产生信号，以及这些信号如何导致受体对血细胞生长和成熟的各种生物学作用。这项研究的结果将有助于理解和治疗涉及血细胞异常生长或功能的疾病，如白血病和炎症性疾病。