Biocontrol properties of pseudozyma flocculosa a natural antagonist of powdery mildew fungi

白粉病真菌天然拮抗剂絮状假酶的生物防治特性

• 批准号: 42188-2006
• 负责人: Bélanger, Richard
• 金额: $ 3.19万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=390455
• 关键词: Biocontrol; properties; pseudozyma; flocculosa; natural

Practical application of biological control of plant pathogens presupposes a thorough understanding of the chemical, biochemical and molecular events underlying such phenomena. This challenge has been the focal point of the research activities carried out in the applicant's  laboratory over the last 16 years. In the previous installment, we have made some important strides toward elucidating key factors governing the biocontrol properties of Pseudozyma flocculosa, a natural antagonist of powdery mildew fungi. Of particular significance, we reported: 1) the structure of an unusual glycolipid accounting for the biocontrol activity of P. flocculosa; 2) the optimization of a genetic transformation system by insertional mutagenesis; 3) the development of a novel technique, reversed intron PCR, that allowed the differentiation of P. flocculosa from other related species and among geographically distinct isolates of P. flocculosa; 4) the antifungal activity of flocculosin against yeast strains of medical importance; and 5) successful expression of foreign genes in P. flocculosa including GFP. In the context of this application and on the basis of our recent progress, the objectives are: 1) to generate and characterize mutants defective for flocculosin production 2) to study and compare potential gene(s) involved in glycolipid production in P. flocculosa and U. maydis; 3) to generate GFP-mutants in both wild type (Wt) and tox- strains of P. flocculosa; 4) to conduct spatio-temporal studies to compare the ecological behavior of P. flocculosa Wt and tox- strains transformed with GFP and 5) to determine the relevance of flocculosin (and related glycolipids) as a fitness determinant in the ecology of P. flocculosa isolates. Fulfillment of these objectives will generate novel and original information with regards to: the genetic determinants that regulate the biocontrol activity of P. flocculosa against powdery mildews and other phyllosphere inhabitants; these results will have far reaching impact into the study of powdery mildew diseases in general and into the study of the genetic, chemical and ecological properties of microbial biocontrol agents, concerns of key importance for their eventual use and manipulation.

植物病原体的生物防治的实际应用的先决条件是彻底了解这种现象背后的化学，生物化学和分子事件。这一挑战一直是申请人实验室在过去16年中开展的研究活动的焦点。在前一部分中，我们已经取得了一些重要的进展，阐明关键因素的生物控制性能的Pseudozyma flocculosa，一种天然拮抗剂的白粉病真菌。特别重要的是，我们报道了：1）一种不寻常的糖脂的结构解释的生物控制活性的絮状委陵菜2）优化遗传转化系统的插入诱变3）一种新的技术，反向内含子PCR的发展，允许区分的其他相关物种和不同地理分离的絮状委陵菜，絮状委陵菜。4）絮凝菌素对具有医学重要性的酵母菌株的抗真菌活性;和5）外源基因在絮凝假单胞菌中的成功表达，包括GFP。在本申请的上下文中，基于我们最近的进展，目的是：1）产生和表征絮凝蛋白产生缺陷的突变体; 2）研究和比较在絮状毛藻和乌藨子中参与糖脂产生的潜在基因。玉米; 3）在絮状假单胞菌的野生型（Wt）和tox-菌株中产生GFP-突变体; 4）进行时空研究以比较用GFP转化的絮状假单胞菌Wt和tox-菌株的生态学行为;和5）确定絮状假单胞菌分离物的生态学中絮状菌素（和相关糖脂）作为适合度决定因子的相关性。这些目标的实现将产生关于以下方面的新颖和原始信息：调节丛毛披碱草对白粉病和其他叶际居民的生物控制活性的遗传决定因素;这些结果将对一般的白粉病疾病的研究和微生物生物防治剂的遗传、化学和生态学特性的研究产生深远的影响，对它们的最终使用和操纵至关重要的关切。