Sensitive, rapid and accurate detection of the emergence of neuraminidase inhibitor resistance by real-time PCR, LCR and

通过实时 PCR、LCR 和

• 批准号: nhmrc : 408114
• 负责人: Dr Bin Wang
• 金额: $ 7.93万
• 依托单位: Sydney West Area Health Service
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Strategic Awards
• 财政年份: 2006
• 资助国家: 澳大利亚
• 起止时间: 2006-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/sensitive-rapid-accurate-pcr-lcr/78381
• 关键词: Sensitive; rapid; accurate; detection; emergence

An influenza pandemic causing by highly pathogenic H5N1 virus may occur in the near future. As a vaccine for H5N1 will not be available in the foreseeable months, antiviral drugs are the only possible choice for prophylaxis and treatment. Currently only two drugs have been clinically proven to be effective against H5N1 strain and the emergence of drug resistant in H5N1 influenza virus has been reported which may significantly hamper the treatment. Understanding and monitoring the emergence of these drug resistant strains during local spreading will be critical in managing an H5N1 influenza pandemic in Australia. In the proposed project, we will develop important diagnostic tools using our world leading Rolling Circle Amplification (RCA) technology for the monitoring of the development and possible transmission of drug resistant influenza strains. Upon finishing the project, at lease three sensitive diagnostic methods will be developed for the detection of the emergence of drug resistance at the very early stage.

由高致病性H5N1病毒引起的流感大流行可能在不久的将来发生。由于在可预见的几个月内还没有H5N1疫苗，抗病毒药物是预防和治疗的唯一可能选择。目前只有两种药物被临床证明对H5N1病毒株有效，并且据报道H5N1流感病毒出现耐药性，这可能会严重阻碍治疗。了解和监测这些耐药菌株在当地传播过程中的出现，对于管理澳大利亚的H5N1流感大流行至关重要。在拟议项目中，我们将利用我们世界领先的滚环扩增（RCA）技术开发重要的诊断工具，以监测耐药性流感菌株的发展和可能的传播。项目完成后，将开发至少三种敏感的诊断方法，用于在早期阶段检测耐药性的出现。