Development of a fiber optic-based exoscope for the in-vivo study of spinal cord disorders

开发用于脊髓疾病体内研究的光纤外窥镜

• 批准号: 350825-2008
• 负责人: Anis, Hanan
• 金额: $ 4.94万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=402229
• 关键词: Development; fiber; optic; based; exoscope

Disorders of the spinal cord are devastating conditions leaving patients with severe lifelong disabilities. It is estimated that ~13,000 individuals suffer paralysis from spinal injuries yearly in North America alone with limited treatment options. Multiple sclerosis is a progressive autoimmune disease affecting the central nervous system and eventually affecting the spinal cord which leads to progressive and irreversible disability, leaving most patients wheelchair-bound. The ultimate objective of this collaborative project is to design and implement a robust fiber-based non-linear optical microscope with miniaturized scanning mechanism and objective lens (< 3mm diameter) that can image spinal cord relatively non-invasively and non-destructively over time in living animals, in 3 dimensions, at sub-micron resolution. The project is divided into 3 specific aims: -Aim 1 (yr 1): to develop a fiber optic based laser scanning CARS/TPEF microscope capable of imaging excised spinal cord tissue in vitro -Aim 2 (yr 2): to modify the system in Aim 1 in order to include multimodal exoscopic capability to enable imaging of intact spinal cord in live rats -Aim 3 (yr 3): to further optimize the system in #2 to image evolution of spinal cord lesions (traumatic, demyelinating) in rodents over time and correlate with MRI data. Modifications will be performed to image mouse cords where axons are genetically encoded to provide green-yellow fluorescence, which together with CARS, will provide a complete picture of the axon-myelin unit.

脊髓疾病是一种毁灭性的疾病，会给患者带来严重的终生残疾。据估计，仅在北美，每年就有约1.3万人因脊柱损伤而瘫痪，治疗选择有限。多发性硬化症是一种进行性自身免疫性疾病，影响中枢神经系统，最终影响脊髓，导致进行性和不可逆转的残疾，使大多数患者不得不坐轮椅。这个合作项目的最终目标是设计和实现一种坚固的基于光纤的非线性光学显微镜，具有小型化的扫描机构和物镜(直径3 mm)，可以在亚微米分辨率下对活体动物的脊髓进行相对非侵入性和非破坏性的三维成像。该项目分为3个具体目标：-目标1(YR 1)：开发能够在体外对切除的脊髓组织进行成像的基于光纤的激光扫描CARS/TPEF显微镜-目标2(YR 2)：对目标1中的系统进行修改，以便包括多模外视能力，以便能够对活体大鼠完整的脊髓进行成像-目标3(年3)：进一步优化#2中的系统，以获得啮齿动物脊髓病变(创伤、脱髓鞘)随时间的演变图像，并与MRI数据进行关联。将进行修改以成像小鼠索，其中轴突被遗传编码以提供绿-黄色荧光，与CARS一起，将提供轴突-髓鞘单位的完整图像。