Environmentally stress-induced suicide module in the biofilm-forming organism streptococcus mutans

生物膜形成生物变形链球菌中环境应激诱导的自杀模块

• 批准号: 355968-2008
• 负责人: Levesque, Céline
• 金额: $ 1.09万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=402795
• 关键词: Environmentally; stress; induced; suicide; module

Biofilms are diverse microbial communities attached to distinct inert or living surfaces. They are ubiquitous and can developed on virtually every natural and man-made surface. The cost to society associated with biofilms is estimated to range in the billions of dollars annually. Biofilms represent a protected mode of growth that allow cells to survive a wide range of environmental challenges. A number of factors have been considered to be responsible for this renowned tenacity of biofilms. Recently, it has been proposed that a genetically determined process of cellular suicide can occur as a feature of the dynamics of some bacterial populations that encounter environmental stresses. Most bacterial chromosomes contain a number of suicide or toxin genes that induce cell growth arrest or cell death. This research program raises the hypothesis that bacteria tightly regulate the expression of their suicide systems to foster bacterial survival of their progeny in the biofilm community. By regulating their suicide systems under various stressful conditions, a subpopulation of cells in the community dies to permit the survival of the population as a whole. The oral bacterium Streptococcus mutans will be used as model organism as this bacterium depends on a biofilm lifestyle for its survival and persistence in its natural ecosystem. The toxic activity of the streptococcal toxin in homologous and heterologous strains will be demonstrated using a protein expression system and the mechanism of action of the toxin characterized. Using quantitative real-time PCR, we will determined the differential gene expression of the suicide system after exposure to several environmental stressors. Finally, reporter systems will be constructed to study the transcriptional activities of the suicidal system in biofilms. These results will provide knowledge of the mechanisms that determine regulated-killing for a better understanding of cell differentiation and death events occurring in biofouling of any surfaces susceptible to microbial contamination. From an applied perspective, the results of this study may lead to the development of novel strategies to control and exploit the biofilm phenomenon in industry and environment technology.

生物膜是附着在不同的惰性或活性表面上的各种微生物群落。它们无处不在，几乎可以在每一个自然和人造表面上开发。与生物膜相关的社会成本估计每年在数十亿美元的范围内。生物膜代表了一种受保护的生长模式，使细胞能够在广泛的环境挑战中生存。许多因素被认为是造成这种著名的生物膜韧性的原因。最近，有人提出，基因决定的细胞自杀过程可能会发生，这是一些遇到环境压力的细菌种群动态的一个特征。大多数细菌染色体含有许多自杀或毒素基因，可诱导细胞生长停滞或细胞死亡。这项研究计划提出了一个假设，即细菌严格调节其自杀系统的表达，以促进其后代在生物膜群落中的细菌存活。通过在各种压力条件下调节自杀系统，群落中的细胞亚群死亡，以使整个种群得以生存。口腔细菌变形链球菌将被用作模式生物，因为这种细菌依赖于生物膜生活方式在其自然生态系统中生存和持久存在。将使用蛋白质表达系统和表征的毒素作用机制来证明链球菌毒素在同源和异源菌株中的毒性活性。利用实时荧光定量PCR技术，我们将确定暴露于几种环境应激后自杀系统的差异基因表达。最后，构建报告系统，研究自杀系统在生物膜中的转录活性。这些结果将提供知识的机制，确定调节杀伤细胞分化和死亡事件发生在任何表面易受微生物污染的生物污损更好地理解。从应用的角度来看，本研究的结果可能会导致新的策略，以控制和利用生物膜现象在工业和环境技术的发展。