Structural characterisation of a natural inhibitor of sporulation bound to its histidine kinase target

与其组氨酸激酶靶标结合的天然孢子形成抑制剂的结构表征

• 批准号: nhmrc : 352434
• 负责人: E/Pr Jules Guss
• 金额: $ 17.4万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2005
• 资助国家: 澳大利亚
• 起止时间: 2005-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/structural-characterisation-natural-kinase-target/99618
• 关键词: Structural; characterisation; natural; inhibitor; sporulation

Many bacteria, including some which are virulent pathogens such as anthrax (Bacillus anthracis), are able to enter a dormant state by forming spores (sporulation). These spores are extremely robust and may persist in the environment buried in the soil for example for hundreds of years. The initiation of sporulation occurs in response to changes in the cellular and environmental conditions which threaten the free replicating existence of the bacterium. The process of sporulation is controlled at the molecular level by a complex signaling relay. It is of course vital for the existence of the organism that control of sporulation is tightly regulated - preventing the onset of spore-formation in any but the desired circumstances. We aim to determine the three-dimensional structures of the molecules involved in this regulated process and how, by interacting with each other, they can pass on the signal to the bacterium to either start or stop the spore forming process. Ultimately, the results of this work might lead to antibacterial agents which could be used to control particularly dangerous strains of bacteria.

许多细菌，包括一些剧毒病原体，例如炭疽杆菌（炭疽杆菌），能够通过形成孢子（孢子形成）进入休眠状态。这些孢子非常坚固，可以在埋在土壤中的环境中持续存在例如数百年。孢子形成的启动是为了响应威胁细菌自由复制存在的细胞和环境条件的变化。孢子形成过程由复杂的信号中继在分子水平上控制。当然，严格调节孢子形成的控制对于生物体的存在至关重要——防止在除所需情况之外的任何情况下孢子形成的发生。我们的目标是确定参与这一调节过程的分子的三维结构，以及它们如何通过相互作用将信号传递给细菌以启动或停止孢子形成过程。最终，这项工作的结果可能会产生抗菌剂，可用于控制特别危险的细菌菌株。