Integrated bioMEMS (Biological Micro Electro Mechanical System) sensor for real-time heat shock protein 70 monitoring in whole organisms

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The goal of the proposed research is to develop a microfluidics and biosensor-based platform for the precise and highly sensitive detection of a key stress-related protein, the heat shock protein 70 (HSP70), in blood. Heatshock proteins are a family of highly conserved proteins synthesized by all organisms and crucial for normal cellular function and for defending cells against stressors. The 70 kilodalton mass HSP (HSP70) is one of the most widely studied member of this protein family and routinely used as an indicator of cellular stress. Recently, several studies in humans correlated blood levels of HSP70 with disease states, including cancer, diabetes, cardiovascular disease and trauma, suggesting the utility of this stress protein measurement as a biomarker of compromised health. We also found that higher serum HSP70 levels reflect chronic stress in