Understanding the structural basis for catalysis and substrate specificity in non-heme diiron medium-chain alkane hydroxylases

了解非血红素二铁中链烷烃羟化酶的催化和底物特异性的结构基础

• 批准号: DP130101930
• 负责人: A/Prof Anna Roujeinikova
• 金额: $ 29.42万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2013
• 资助国家: 澳大利亚
• 起止时间: 2013-09-09 至 2016-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP130101930
• 关键词: Understanding; structural; basis; catalysis; substrate

This work will determine the molecular basis for catalysis and specificity of non-heme diiron medium-chain alkane hydroxylases by obtaining the first structure of such an enzyme. This pivotal knowledge will drive enzyme engineering for applications in biodegradation of spilt oils and biosynthesis of pharmaceuticals and high-cost chemicals.

本工作将通过获得非血红素二铁中链烷烃羟化酶的首个结构，确定非血红素二铁中链烷烃羟化酶催化和特异性的分子基础。这一关键知识将推动酶工程应用于溢出油的生物降解和药物和高成本化学品的生物合成。