Ultra-low temperature freezer for storage of biological samples

用于储存生物样本的超低温冰箱

• 批准号: 374784-2009
• 负责人: Mielke, John
• 金额: $ 1.1万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412013
• 关键词: Ultra; low; temperature; freezer; storage

The applicants behind the current proposal use animal models to answer basic questions about the way in which the nervous and immune systems work. As part of their studies, both laboratories withdraw tissue samples from animals that have been used in nutritional or exercise-related experiments. The isolated samples contain the very basic building blocks of life (DNA and protein), which can be analysed to show whether changes have been caused by the different experiments. For example, the possibility that eating certain foods affects our behaviour can be studied by looking for diet-induced changes in the levels of proteins known to be important for brain function. Due to the complex design of animal experiments, the importance of making the most of tissue taken from animals, and the fact that many experiments need to be done several times, the collected samples need to be stored in a fashion that will allow them to be kept in a stable condition for long periods. The best way to maintain tissue samples in a good state over a long period of time is to keep them at a very low temperature. By doing so, the breakdown of the samples that would normally occur can be avoided, and the tissue can be analysed on many occasions. As a result, the quality of data that can be gathered will be improved, and the number of animals that need to be used will be reduced.

目前提案背后的申请人使用动物模型来回答有关神经和免疫系统工作方式的基本问题。作为研究的一部分，两个实验室都从用于营养或运动相关实验的动物身上提取组织样本。分离的样本包含生命的基本组成部分（DNA和蛋白质），可以对其进行分析，以显示不同的实验是否引起了变化。例如，吃某些食物影响我们行为的可能性可以通过寻找饮食引起的蛋白质水平变化来研究，这些蛋白质对大脑功能很重要。由于动物实验的复杂设计，充分利用动物组织的重要性，以及许多实验需要多次进行的事实，收集的样本需要以一种允许它们长期保持稳定状态的方式储存。长期保持组织样本处于良好状态的最佳方法是将其保持在非常低的温度下。通过这样做，可以避免通常会发生的样品分解，并且可以在许多情况下分析组织。因此，可以收集的数据质量将得到提高，需要使用的动物数量将减少。