Plant CO2 gas exchange and fluorescence system

植物CO2气体交换和荧光系统

• 批准号: 374953-2009
• 负责人: Huner, Norman
• 金额: $ 3.6万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412045
• 关键词: Plant; CO2; gas; exchange; fluorescence

Chlorophyll a fluorescence combined with CO2 gas exchange is now a technique of choice to assess photosynthetic performance and the effects of environmental stress in plants and algae. The Li-Cor 6400XRT represents state-of-the-art instrumentation for this purpose and will support three independent research programmes in the general area of plant stress and development. First, the ability to combine measurements of in vivo CO2 gas exchange with concomitant measurements of chlorophyll fluorescence is crucial to elucidating the mechanism underlying the regulation of stress-induced leaf variegation in the model plant, Arabidopsis thaliana. In addition, in vivo CO2 gas exchange and chlorophyll fluorescence measurements coupled with gene expression measurements are critical to understanding the mechanism underlying the acclimation of specific cereal cultivars to elevated CO2 and warm temperatures typically associated with global warming and climate change. Second, Dr. Macfie's research programme is focussed on the elucidation of the mechanisms by which plants respond to potentially toxic metal ions. This includes identification of the biochemical and molecular mechanism(s) in operation, the corresponding signalling pathways and the genes or gene families involved. Metal stress induced changes in biomass accumulation should be reflected in reductions in photosynthetic capacity and photosynthetic efficiency. In turn, this should be reflected in alterations in CO2 assimilation rates, PSII photochemical efficiency and energy partitioning as measured by in vivo chlorophyll a fluorescence and CO2 gas exchange. Third, Dr. Henry's current research focuses on plant and natural ecosystem responses to the combined effects of climate warming and atmospheric nitrogen deposition, two ecologically important global change factors that are projected to increase over the next century. CO2 assimilation combined with chlorophyll a fluorescence data will help in the prediction of the future productivity and functionality of temperate ecosystems in response to global environmental change, and will contribute to the mechanistic framework underlying feedbacks between the atmosphere and biotic systems in climate change models.

叶绿素a荧光结合二氧化碳气体交换现在是评估植物和藻类光合作用性能和环境胁迫影响的首选技术。Li-Cor 6400XRT代表了为此目的最先进的仪器，并将支持植物胁迫和发育一般领域的三个独立研究项目。首先，结合体内二氧化碳气体交换测量和叶绿素荧光测量的能力，对于阐明模式植物拟南芥（Arabidopsis thaliana）中应激诱导的叶片杂色调节机制至关重要。此外，体内CO2气体交换和叶绿素荧光测量与基因表达测量相结合，对于了解特定谷物品种对二氧化碳升高和温暖温度的适应机制至关重要，这些升高和温暖温度通常与全球变暖和气候变化有关。其次，麦克菲博士的研究重点是阐明植物对潜在有毒金属离子的反应机制。这包括在操作中的生化和分子机制的识别，相应的信号通路和所涉及的基因或基因家族。金属胁迫引起的生物量积累变化应反映在光合能力和光合效率的降低上。反过来，这应该反映在CO2同化率、PSII光化学效率和能量分配（通过体内叶绿素a荧光和CO2气体交换测量）的变化上。第三，亨利博士目前的研究重点是植物和自然生态系统对气候变暖和大气氮沉降的综合影响的反应，这两个生态上重要的全球变化因素预计将在下个世纪增加。二氧化碳同化与叶绿素a荧光数据的结合将有助于预测温带生态系统对全球环境变化的未来生产力和功能，并将有助于在气候变化模式中建立大气和生物系统之间反馈的机制框架。