Recombinant metalloproteins and living cells for chemical research

研究化学和生物学之间的界面（“化学生物学”）利用化学的力量来帮助理解生物系统固有的复杂性。化学生物学是一个令人兴奋的研究领域，现在是化学科学研究的主导主题。这一领域的竞争性研究需要常规使用培养和分离各种细胞系以及诱导和合成重组金属蛋白的细胞孵育所需的设备。这个建议是关于购买这个设备的，这对UWO化学系的大量研究人员来说是至关重要的。有了这个新设备，申请人将进行大量的研究。利用重组金属蛋白，Stillman将探索金属依赖蛋白折叠和金属依赖代谢功能的细节，特别是在神经生理学方面。Konermann将研究蛋白质错误折叠作为淀粉样蛋白聚集的原因的影响，淀粉样蛋白聚集被认为在阿尔茨海默病和脑生理学中起着至关重要的作用。界面研究的一个非常活跃的领域涉及整个活细胞和细胞膜的化学性质。吉利斯将开发新型抗生素，通过破坏细菌细胞膜起作用。活细胞的分子水平研究是Norton， lagugn<s:1> - labarthet和Ding研究的一个主要目标，其中显微镜的化学和技术发展将用于活细胞特性的新研究。这些发展将导致新的成像模式能够纳米空间分辨率，高时间分辨率和单分子检测。丁和克拉茨将研究化学物质对活细胞代谢活动的影响。总而言之，所要求的设备将为大量研究人员提供迄今为止无法获得的设施，允许重组蛋白的合成和细胞模型的生长，这将导致我们对生物分子和结构及其功能之间关系的理解取得令人兴奋的进展。

Research at the interface between chemistry and biology ("chemical biology") utilizes the power of chemistry to help understand the complexity inherent in biological systems. Chemical biology is an exciting area of study that is now a dominant theme in research in the chemical sciences. Competitive research in this area requires routine access to the equipment required for the culture and isolation of a variety of cell lines and the incubation of cells for induction and synthesis of recombinant metalloproteins. This proposal concerns purchase of this equipment, which is of crucial importance to a large number of researchers in the UWO Chemistry Department. With this new equipment, the applicants will carry out a large number of research studies. Using recombinant metalloproteins Stillman will probe the details of metal-dependent protein folding and metal-dependent metabolic function, particularly in neurophysiology and Konermann will study the effects of misfolding of proteins as the cause of amyloid protein aggregates, which are considered to play a vital role in Alzheimer's disease, and in brain physiology. An extremely active area of interfacial research concerns the chemical properties of whole living cells and cell membranes. Gillies will develop novel antibiotics, which act by disrupting the membranes of bacterial cells. The molecular level study of live cells is a major objective in the research of Norton, Lagugné-Labarthet and Ding in which chemical and technical developments in microscopy will be used for novel research on the properties of living cells. These developments will lead to new imaging modalities capable of nanometer spatial resolution, high temporal resolutions, and single molecule detection. Ding and Kraatz will study the effects of chemicals on the metabolic activities of living cells. In summary, the requested equipment will provide a hitherto unavailable facility for a large number of researchers allowing the synthesis of recombinant proteins and growth of cell models that will lead to exciting progress to be made in our understanding of the relationships between biological molecules and structures, and their functions.