Electrophysiology suite to characterize visual function in fishes

用于表征鱼类视觉功能的电生理学套件

• 批准号: 375076-2009
• 负责人: Allison, WilliamTed
• 金额: $ 4.98万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412075
• 关键词: Electrophysiology; suite; characterize; visual; function

This proposal requests support for an electrophysiology suite to measure the visual abilities of fish. A long history of research has attempted to understand the evolution of fish vision by comparing fish that live in different environments, or by testing visual system efficiencies with varied backgrounds/stimuli. Here we focus instead on testing hypotheses regarding the adaptive value of characters by altering retinal features through gene knockdown, mis-expression and transgenesis in zebrafish. The electrophysiology equipment requested here is a custom designed suite of recording equipment with an optical bench for delivery of photic stimuli. Because fish are able to visually discriminate visual targets containing ultraviolet and polarized light, emphasis has been placed on designing optics to maximize UV output. The optical bench thus includes high-wattage lamps and equipment to control the wavelength, duration, calibration and transmission of stimuli. Electrophysiological equipment includes a Faraday cage, amplifiers and equipment to place electrodes on the surface of the eye of larval fish. Software will be customized to control stimulus delivery and integrate data-acquisition. The equipment allows us to integrate data of developmental mechanisms/morphology with recordings of visual sensitivity. The equipment will be used to investigate:

该提案要求支持电生理学套件来测量鱼的视觉能力。长期以来的研究试图通过比较生活在不同环境中的鱼类，或通过测试视觉系统在不同背景/刺激下的效率，来了解鱼类视觉的进化。在这里，我们的重点是通过基因敲除、错误表达和转基因在斑马鱼中改变视网膜特征来测试有关特征适应价值的假设。这里要求的电生理设备是一套定制设计的记录设备，带有光学工作台，用于传递光刺激。由于鱼类能够在视觉上区分含有紫外线和偏振光的视觉目标，因此重点放在设计光学器件以最大化紫外线输出上。因此，光学工作台包括高瓦数的灯和控制波长、持续时间、校准和传输刺激的设备。电生理设备包括法拉第笼、放大器和将电极放置在幼鱼眼睛表面的设备。软件将被定制来控制刺激传输和集成数据采集。该设备使我们能够将发育机制/形态学数据与视觉灵敏度记录相结合。该设备将用于调查：