Automated DNA synthesizer

自动化DNA合成仪

• 批准号: 375270-2009
• 负责人: Sleiman, Hanadi
• 金额: $ 6.56万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412121
• 关键词: Automated; DNA; synthesizer

Our research group focuses on the development of DNA-mediated methods to pattern materials with high precision on the nanometer scale. Towards this goal we have examined the use of synthetic molecules to direct DNA self-assembly. This has resulted in a range of stimuli-responsive DNA materials with fine positioning of each component (Science, 2008, 321, 1795). The present proposal describes the development of these new biomaterials towards a number of applications: 1. Gold nanoparticle assemblies as substrates for optimized focusing of light to nanoscale volumes, and applications in the amplified detection of DNA via surface enhanced Raman scattering. 2. Well-defined 2D- and 3D-metal nucleic acid frameworks for applications in artificial photosynthesis and multimetallic catalysis. 3. 3D-DNA capsules and nanotubes as chemical biology tools to modulate the stability and activity of encapsulated proteins, and to deliver toxins selectively to tumor cells.

我们的研究小组专注于开发DNA介导的方法，以在纳米尺度上实现高精度的材料图案化。 为了实现这一目标，我们已经研究了使用合成分子来指导DNA自组装。 这导致了一系列刺激响应性DNA材料，其中每个组分具有精细定位（Science，2008，321，1795）。 本提案描述了这些新的生物材料的发展对一些应用：1。金纳米粒子组件作为优化光聚焦到纳米级体积的基底，以及通过表面增强拉曼散射在DNA放大检测中的应用。 2.定义明确的2D和3D金属核酸框架，用于人工光合作用和多金属催化。3. 3D-DNA胶囊和纳米管作为化学生物学工具来调节封装蛋白质的稳定性和活性，并将毒素选择性地递送到肿瘤细胞。