The effect of cyclic strain and pressure on endothelial cell response

心脏病、中风、动脉瘤和瓣膜疾病等心血管疾病被认为部分是细胞对生物力学力的反应。 我的实验室已经开发了使用三维组织培养模型的技术，以更好地研究内皮细胞，白细胞和血小板对现实血流动力学的反应。目前，这些模型仅限于研究剪切应力作为一个刺激，由于我们的假设刚性壁力学。 在体内，动脉壁是顺应性的，特别是在冠状动脉的情况下，在心动周期期间被周围组织牵拉和拉伸。 所提出的工作的目的是增加循环应变和遵守我们现有的动态细胞培养模型。具体来说，我们将回答以下问题：1。血管顺应性和周期性应变是否改变内皮细胞的局部反应？ 2.血液成分与内皮的连接和附着是否受到顺应性和周期性应变的影响？ 这项研究将提供更好的了解动脉粥样硬化，并最终导致更好的治疗方案。

Cardiovascular diseases such as heart attacks, strokes, aneurysms and valvular disorders are believed to be partly a cellular response to biomechanical forces. My laboratory has developed the techniques to use three dimensional tissue culture models to better investigate the response of endothelial cells, leukocytes and platelets to realistic hemodynamics. Currently these models are limited to the study of shear stress as a stimulus owing to our assumption of rigid wall mechanics. In vivo, the artery wall is compliant and, particularly in the case of coronary arteries, pulled and stretched by surrounding tissue during the cardiac cycle. The objective of the proposed work is to add cyclic strain and compliance to our existing dynamic cell culture models. Specifically, we will answer the questions: 1. Does vessel compliance and cyclic strain alter the local response of endothelial cells? 2. Is the tethering and attachment of blood components to the endothelium affected by compliance and cyclic strain? This research will provide a better understanding of atherosclerosis and ultimately lead to better treatment options.