Analysis of lasp function in myofibril assembly

肌原纤维组装中lasp功能的分析

• 批准号: 298534-2009
• 负责人: Schoeck, Frieder
• 金额: $ 2.55万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=427624
• 关键词: Analysis; lasp; function; myofibril; assembly

We propose to investigate the protein Lasp, which is essential for proper muscle function. The smallest contractile units of muscles have two filament systems, the thick and thin filaments that slide past each other to generate contractile force. A mutation in Lasp results in shorter thin filaments, and therefore impaired muscle function, as the contractile force that can be generated with shorter thin filaments is smaller. We will investigate how Lasp regulates thin filament length with genetic, biochemical, and advanced imaging methods, such as live imaging of thin filament turnover. These basic cell biological studies will shed light on important aspects of muscle function in development and disease.

我们建议研究Lasp蛋白，它是正常肌肉功能所必需的。最小的肌肉收缩单位有两个细丝系统，粗细细丝相互滑动以产生收缩力。Lasp的突变导致更短的细纤维，因此肌肉功能受损，因为更短的细纤维可以产生的收缩力更小。我们将研究Lasp如何通过遗传、生化和先进的成像方法（如细丝周转的实时成像）调节细丝长度。这些基本的细胞生物学研究将揭示肌肉功能在发育和疾病中的重要方面。