Scanning electrochemical microscopy studies of cells: transport, viability and communication

细胞的扫描电化学显微镜研究：运输、活力和通讯

• 批准号: 312503-2008
• 负责人: Mauzeroll, Janine
• 金额: $ 2.62万
• 依托单位: Université du Québec à Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=428252
• 关键词: Scanning; electrochemical; microscopy; studies; cells

Scanning Electrochemical Microscopy (SECM) is useful because it probes the chemical or biochemical activity of a surface. It does so by measuring a current at a sensor that is moved towards or over a sample using precise positioners. This specific detection method can detect small concentrations of compounds being absorbed or released from cancer cells. To apply SECM to live cell studies, cells have to be immobilized on a solid support. The sensor is positioned non-invasively a few microns above it. The cells are then stimulated by a chemical to release compounds that can directly be detected by SECM. From such measurements and using numerical simulations, the rates of transport and kinetic parameters related to these events can be extracted

扫描电化学显微镜 (SECM) 非常有用，因为它可以探测表面的化学或生化活性。它通过使用精确定位器测量朝向样品或在样品上方移动的传感器处的电流来实现这一点。这种特定的检测方法可以检测癌细胞吸收或释放的小浓度化合物。要将 SECM 应用于活细胞研究，细胞必须固定在固体支持物上。传感器以非侵入方式放置在其上方几微米处。 然后，细胞受到化学物质的刺激，释放出可直接被 SECM 检测到的化合物。通过此类测量并使用数值模拟，可以提取与这些事件相关的传输速率和动力学参数