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Scanning electrochemical microscopy studies of cells: transport, viability and communication

Scanning electrochemical microscopy studies of cells: transport, viability and communication
细胞的扫描电化学显微镜研究:运输、活力和通讯
批准号:
312503-2008
负责人:
Mauzeroll, Janine
金额:
$2.62万
依托单位国家:
加拿大
项目类别:
Discovery Grants Program - Individual
财政年份:
2009
资助国家:
加拿大
项目状态:
已结题
起止时间:
2009-01-01 至 2010-12-31

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英文摘要
Scanning Electrochemical Microscopy (SECM) is useful because it probes the chemical or biochemical activity of a surface. It does so by measuring a current at a sensor that is moved towards or over a sample using precise positioners. This specific detection method can detect small concentrations of compounds being absorbed or released from cancer cells. To apply SECM to live cell studies, cells have to be immobilized on a solid support. The sensor is positioned non-invasively a few microns above it. The cells are then stimulated by a chemical to release compounds that can directly be detected by SECM. From such measurements and using numerical simulations, the rates of transport and kinetic parameters related to these events can be extracted
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国内基金
海外基金
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