Development of a fiber optic-based exoscope for the in-vivo study of spinal cord disorders

开发用于脊髓疾病体内研究的光纤外窥镜

• 批准号: 350825-2008
• 负责人: Anis, Hanan
• 金额: $ 5.17万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=431332
• 关键词: Development; fiber; optic; based; exoscope

Disorders of the spinal cord are devastating conditions leaving patients with severe lifelong disabilities. It is estimated that ~13,000 individuals suffer paralysis from spinal injuries yearly in North America alone with limited treatment options. Multiple sclerosis is a progressive autoimmune disease affecting the central nervous system and eventually affecting the spinal cord which leads to progressive and irreversible disability, leaving most patients wheelchair-bound. The ultimate objective of this collaborative project is to design and implement a robust fiber-based non-linear optical microscope with miniaturized scanning mechanism and objective lens (< 3mm diameter) that can image spinal cord relatively non-invasively and non-destructively over time in living animals, in 3 dimensions, at sub-micron resolution. The project is divided into 3 specific aims: -Aim 1 (yr 1): to develop a fiber optic based laser scanning CARS/TPEF microscope capable of imaging excised spinal cord tissue in vitro -Aim 2 (yr 2): to modify the system in Aim 1 in order to include multimodal exoscopic capability to enable imaging of intact spinal cord in live rats -Aim 3 (yr 3): to further optimize the system in #2 to image evolution of spinal cord lesions (traumatic, demyelinating) in rodents over time and correlate with MRI data. Modifications will be performed to image mouse cords where axons are genetically encoded to provide green-yellow fluorescence, which together with CARS, will provide a complete picture of the axon-myelin unit.

脊髓疾病是一种毁灭性的疾病，会使患者终身残疾。据估计，仅在北美，每年就有约13，000人因脊髓损伤而瘫痪，治疗选择有限。多发性硬化症是一种进行性自身免疫性疾病，影响中枢神经系统，最终影响脊髓，导致进行性和不可逆的残疾，使大多数患者坐轮椅。 这个合作项目的最终目标是设计和实现一个强大的基于光纤的非线性光学显微镜，具有小型化的扫描机制和物镜透镜（直径<3 mm），可以相对非侵入性和非破坏性地随着时间的推移在活体动物的脊髓成像，在3维，亚微米分辨率。该项目分为3个具体目标：-目标1（第1年）：开发一种基于光纤的激光扫描汽车/TPEF显微镜，能够在体外对切除的脊髓组织进行成像-目标2（第2年）：修改目标1中的系统，以包括多模式外镜功能，从而能够对活体大鼠的完整脊髓进行成像-目标3（第3年）：进一步优化#2中的系统，以随时间对啮齿动物的脊髓病变（创伤性、脱髓鞘）的演变进行成像，并与MRI数据相关联。将进行修改，以成像小鼠脊髓，其中轴突被遗传编码，以提供绿黄色荧光，这与汽车一起，将提供轴突髓鞘单位的完整图片。