Incubators for cell culture

点击翻译按钮获取中文摘要

The requested instrumentation will be used primarily for the controlled culture of mammalian cells, an essential procedure for the researchers submitting the application and for others who work in the Department of Biology. The equipment will be employed for characterization of DNA, RNA and proteins in cells during normal growth and when exposed to environmental and physiological stress, including disease. Dr. MacRae analyzes gene expression and protein synthesis during Artemia embryo development and diapause, a state of physiological dormancy and enhanced stress tolerance. Protein structure/function relationships and the contribution of molecular chaperones to cell survival during diapause are considered in his work. Dr. Pohajdak studies intracellular transport of proteins in natural killer cells, major mediators of immunity. Much of the work entails examination of protein-protein interactions involving the adaptor CASP (Cytohesin Associated Scaffolding Protein) which interacts with members of the cytohesin/ARNO family. The research of both applicants represents fundamental contributions to cell, molecular and developmental biology, and there are many applications. Dr. MacRae's research relates to the role of molecular chaperones in heart disease, cataract, and neurological disorders. Examination of diapause may reveal how insects resist stress, this of importance in agriculture and forestry where these organisms are often pests. A greater understanding of diapause will enhance the use of Artemia as feed in aquaculture. Dr. Pohajdak's research contributes to our understanding of the immune system in recognization and elimination of cancer cells. Clearly, there is potential to make discoveries which have direct consequences to health and commerce through use of the incubators sought herein. The equipment will also facilitate experiments addressing basic questions which are the cornerstone of many research programs, thus placing the applicants at a definite advantage in the effective use of grant funds and the ability to conduct modern experimentation in several biological disciplines.