Cryosectionning unit for an ultramicrotome to facilitate immunolabeling for electron microscopy and protein localization using mass spectrometry

用于超薄切片机的冷冻切片装置,可促进电子显微镜免疫标记和使用质谱法进行蛋白质定位

基本信息

  • 批准号:
    390535-2010
  • 负责人:
  • 金额:
    $ 3.89万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Research Tools and Instruments - Category 1 (<$150,000)
  • 财政年份:
    2009
  • 资助国家:
    加拿大
  • 起止时间:
    2009-01-01 至 2010-12-31
  • 项目状态:
    已结题

项目摘要

Five co-applicants on this proposal would like to purchase an attachment for our existing ultramicrotome, which was funded by NSERC in 2003, to allow us to cut very thin slices of previously frozen tissue while maintaining the frozen state. This technique and the specialized equipment that is necessary to perform the technique is extremely useful for identifying specific molecules in cells and tissues using antibodies tagged with a label that can be seen under a transmission electron microscope. As a result, the location of the molecule of interest can be pin-pointed with very high accuracy within cells and subcellular compartments. By preserving the molecular components of cells and tissues by freezing, rather than with chemical fixatives, molecules of interest are less likely to be damaged beyond recognition by antibodies, so sensitivity of the labeling technique is greatly improved. Furthermore, the technique does not require the use of detergents to make cell membranes permeable to antibodies, a necessary step of conventional antibody-labeling procedures that drastically diminishes the quality of cellular ultrastructure. The research that will be facilitated by the requested equipment includes: 1) analysis of the effects of maternal stress on the developing nervous system of offspring, 2) study of neural circuits in the mammalian eye, 3) study of the defensive responses of poplar trees as induced by herbivore attacks, and 4) study of the developing nervous system in animals that have a larval stage in the life cycle. In addition, the equipment will be used to explore the use of very thin frozen sections for a mass spectrometry technique, uniquely available at the University of Victoria - Genome BC Proteomics facility in Victoria. This technique allows proteins to be identified and localized with high accuracy within cells and tissues and will be applied to a study of seed development in economically important conifers. A skilled technician employed full time in our Electron Microscopy Laboratory would provide expert training for graduate students in the method of cutting frozen, ultrathin sections.
该提案的五位共同申请人希望为我们现有的超薄切片机购买一个附件,该附件由NSERC于2003年资助,使我们能够在保持冷冻状态的同时切割非常薄的先前冷冻组织切片。该技术和执行该技术所需的专用设备对于使用标记有标签的抗体识别细胞和组织中的特定分子非常有用,该标签可以在透射电子显微镜下看到。因此,可以在细胞和亚细胞区室内以非常高的精度精确定位感兴趣分子的位置。通过冷冻保存细胞和组织的分子组分,而不是用化学固定剂,感兴趣的分子不太可能被破坏而无法被抗体识别,因此标记技术的灵敏度大大提高。此外,该技术不需要使用去污剂来使细胞膜对抗体可渗透,这是常规抗体标记程序的必要步骤,其大大降低了细胞超微结构的质量。所要求的设备将有助于进行的研究包括:1)分析母体压力对后代神经系统发育的影响,2)研究哺乳动物眼睛的神经回路,3)研究白杨受到草食动物攻击后的防御反应,4)研究生命周期中处于幼虫阶段的动物的神经系统发育。此外,该设备将用于探索使用非常薄的冷冻切片进行质谱分析技术,这在维多利亚大学-维多利亚的基因组BC蛋白质组学设施中是独一无二的。这种技术允许蛋白质被识别和定位在细胞和组织内的高精度,并将被应用于种子发育的研究在经济上重要的针叶树。我们的电子显微镜实验室全职雇用一名熟练的技术人员,为研究生提供冷冻切片切割方法的专家培训。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Page, Louise其他文献

Effect of the Growth Assessment Protocol on the DEtection of Small for GestatioNal age fetus: process evaluation from the DESiGN cluster randomised trial.
  • DOI:
    10.1186/s13012-022-01228-1
  • 发表时间:
    2022-09-05
  • 期刊:
  • 影响因子:
    7.2
  • 作者:
    Relph, Sophie;Coxon, Kirstie;Vieira, Matias C.;Copas, Andrew;Healey, Andrew;Alagna, Alessandro;Briley, Annette;Johnson, Mark;Lawlor, Deborah A.;Lees, Christoph;Marlow, Neil;McCowan, Lesley;McMicking, Jessica;Page, Louise;Peebles, Donald;Shennan, Andrew;Thilaganathan, Baskaran;Khalil, Asma;Pasupathy, Dharmintra;Sandall, Jane
  • 通讯作者:
    Sandall, Jane
Prescribing for pregnancy: managing prescribing for women with mental health diagnoses.
  • DOI:
    10.1136/dtb.2019.000006
  • 发表时间:
    2020-01-01
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Page, Louise
  • 通讯作者:
    Page, Louise

Page, Louise的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Page, Louise', 18)}}的其他基金

Developmental Modules & Evolvability: The Gastropod Foregut as a Model
发展模块
  • 批准号:
    RGPIN-2015-06484
  • 财政年份:
    2019
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Developmental Modules & Evolvability: The Gastropod Foregut as a Model
发展模块
  • 批准号:
    RGPIN-2015-06484
  • 财政年份:
    2018
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Developmental Modules & Evolvability: The Gastropod Foregut as a Model
发展模块
  • 批准号:
    RGPIN-2015-06484
  • 财政年份:
    2017
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Developmental Modules & Evolvability: The Gastropod Foregut as a Model
发展模块
  • 批准号:
    RGPIN-2015-06484
  • 财政年份:
    2016
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Developmental Modules & Evolvability: The Gastropod Foregut as a Model
发展模块
  • 批准号:
    RGPIN-2015-06484
  • 财政年份:
    2015
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Evolution of development among gastropod molluscs
腹足类软体动物的发育演变
  • 批准号:
    138169-2009
  • 财政年份:
    2014
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Evolution of development among gastropod molluscs
腹足类软体动物的发育演变
  • 批准号:
    138169-2009
  • 财政年份:
    2012
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Evolution of development among gastropod molluscs
腹足类软体动物的发育演变
  • 批准号:
    138169-2009
  • 财政年份:
    2011
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Evolution of development among gastropod molluscs
腹足类软体动物的发育演变
  • 批准号:
    138169-2009
  • 财政年份:
    2010
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual
Evolution of development among gastropod molluscs
腹足类软体动物的发育演变
  • 批准号:
    138169-2009
  • 财政年份:
    2009
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Discovery Grants Program - Individual

相似国自然基金

基于压力敏感肾单位微流控芯片的肾上皮细胞CAT1-mTOR通路在梗阻性肾损伤中的作用机制研究
  • 批准号:
    82370678
  • 批准年份:
    2023
  • 资助金额:
    49.00 万元
  • 项目类别:
    面上项目

相似海外基金

Neurovascular unitにおけるてんかん原生機序と抗てんかん薬の効果
致癫痫机制和抗癫痫药物对神经血管单位的影响
  • 批准号:
    24K12268
  • 财政年份:
    2024
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Collaborative Research: SHF: Medium: Enabling Graphics Processing Unit Performance Simulation for Large-Scale Workloads with Lightweight Simulation Methods
合作研究:SHF:中:通过轻量级仿真方法实现大规模工作负载的图形处理单元性能仿真
  • 批准号:
    2402804
  • 财政年份:
    2024
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Standard Grant
Functional-unit-based hierarchical nanocomposites for sustainable future
基于功能单元的分层纳米复合材料促进可持续未来
  • 批准号:
    FT230100436
  • 财政年份:
    2024
  • 资助金额:
    $ 3.89万
  • 项目类别:
    ARC Future Fellowships
Bain unitに着目したベイナイト鋼の低温脆性抑制機構の解明
以贝恩单元为中心阐明贝氏体钢低温脆性抑制机制
  • 批准号:
    24K17181
  • 财政年份:
    2024
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Neuro-Vascular Unitの構築を基盤とした、もやもや病の病態解明
基于神经血管单元的构建阐明烟雾病的病理学
  • 批准号:
    24K18427
  • 财政年份:
    2024
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
SBIR Phase I: Oscillator Processing Unit - Physical Reservoir Computing on the Edge
SBIR 第一阶段:振荡器处理单元 - 边缘物理油藏计算
  • 批准号:
    2335448
  • 财政年份:
    2024
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Standard Grant
Subtractive Manufacturing Unit (SMU)
减材制造单元 (SMU)
  • 批准号:
    524727162
  • 财政年份:
    2023
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Major Research Instrumentation
An AI-driven clinical washbasin unit that automatically disinfects pathogens, reduces aerosols and decreases healthcare-acquired infections by 70%
%20人工智能驱动%20临床%20洗脸盆%20单位%20%20自动%20消毒%20病原体,%20减少%20气溶胶%20和%20减少%20医疗保健获得性%20感染%20by%2070%
  • 批准号:
    83001507
  • 财政年份:
    2023
  • 资助金额:
    $ 3.89万
  • 项目类别:
    Innovation Loans
Project 3: 3-D Molecular Atlas of cerebral amyloid angiopathy in the aging brain with and without co-pathology
项目 3:有或没有共同病理的衰老大脑中脑淀粉样血管病的 3-D 分子图谱
  • 批准号:
    10555899
  • 财政年份:
    2023
  • 资助金额:
    $ 3.89万
  • 项目类别:
Core B: Clinical Trials Core
核心 B:临床试验核心
  • 批准号:
    10554476
  • 财政年份:
    2023
  • 资助金额:
    $ 3.89万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了