Gene induction analyses

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During virus infections, the immune system with its complex network composed of many immune cells need to signal the right messages to eradicate the foreign pathogen. These messages are what we refer to as cytokines, which are mainly cellular proteins. These cytokines interact with cells of the immune system to facilitate the body's response to infection. A controlled cytokine production is indicative of a healthy immune response that should resolve the infection. On the other hand if the cytokines are produced at very high levels, they could lead to tissue damage due to an over active immune response. Conversely, a low production of the cytokines would imply immunosuppression and may be associated with the inability to clear the infection in a timely manner. Cytokines are released by immune cells into the circulation and can be measured by different means. A common approach to measure the induction of these cytokines is to employ a technique known as the polymerase chain reaction (PCR). This technique allows for the detection of cytokine gene activation. This is a complex but very reliable technique that necessitates specialized equipments that we currently need in our laboratory. To accomplish this, we need to acquire a Microcentrifuge needed for sample preparation at 4°C. We also require Dual Fast Reaction Thermal Cycler which will allow to perform more tests and optimize our reactions at two different settings simultaneously. Thus, the equipments we are asking for will enhance the training and progress of the trainees' research. In particular, it will improve the training of our current team comprised of a post-doc researcher, a master student, two PhD graduate students and one undergraduate student.