Flow Cytometry Cell Sorting Analysis Research Platform.

流式细胞术细胞分选分析研究平台。

• 批准号: 472604-2015
• 负责人: Basta, Sameh
• 金额: $ 10.92万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564790
• 关键词: Flow; Cytometry; Cell; Sorting; Analysis

Fluorescence Activated Cell Sorting (FACS) is routinely used to enrich for a specific cell type from a heterogeneous cell suspension. FACS sorting relies on the binding of fluorescence-tagged antibodies to specific biomarkers such as receptors on the surface of the cell. The characteristic fluorescent properties activated by laser emissions readily identify cells of interest and permits their separation at exceptional purity. This is an effective and rapid approach to isolate many biological cell types and will catalyze much deeper levels of our ongoing biological research that are aimed at integrating intracellular signaling events with functions in the specific cell types of interest. The dual laser Sony SH800 high-speed FACS with a biosafety cabinet is requested to support NSERC-funded research work in the fields of and cell biology, genetics, biotechnology, infection, immunity, and inflammation. This equipment will sort cells that require biohazard level II containment, a feature not currently available at Queen's University. The biosafety feature is essential to limit the exposure of highly qualified personnel (HQP) to infectious agents and to maintain the integrity of the biological samples for transplantation or culture following collection.

荧光激活细胞分选(FACS)通常用于从异种细胞悬液中浓缩特定类型的细胞。FACS分选依赖于荧光标记的抗体与特定生物标记物的结合，例如细胞表面的受体。激光发射激活的特征荧光特性很容易识别感兴趣的细胞，并使它们能够以极高的纯度进行分离。这是一种有效和快速分离多种生物细胞类型的方法，并将催化我们正在进行的生物学研究的更深层次，旨在将细胞内信号事件与感兴趣的特定细胞类型的功能相结合。带有生物安全柜的双激光索尼SH800高速FACS被要求支持NSERC资助的细胞生物学、遗传学、生物技术、感染、免疫和炎症领域的研究工作。这种设备将对需要二级生物危害遏制的细胞进行分类，这是皇后大学目前没有的一项功能。生物安全特征对于限制高资质人员(HQP)接触传染病病原体以及保持用于移植或采集后培养的生物样本的完整性至关重要。