CE-Uv-MS for bioorganic, medicinal, and materials chemistry

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This application requests funding for a capillary electrophoresis system coupled to Uv-Visible and mass spectrometry detectors (CE-Uv-MS). This technique combines the short analysis time and extraordinarily high separation efficiency of capillary electrophoresis (CE-Uv) with the molecular weight and structural information derived from the mass spectrometry detector (MS). The technique successfully separates and analyzes charged compounds such as peptides, aminoglycoside antibiotics, nucleic acids, and coenzyme A derivatives. The equipment applied for is essential to the research programs of 4 PIs in the Dept. of Chemistry. Many other PIs have expressed the need for access to this instrument. The research projects enhanced or enabled by the CE-Uv-MS, include: 1) fingerprinting of plant extracts for clinical studies and identification of the active ingredients; 2) engineering of P450 enzymes; 3) identification of protein targets of arsenical antileukemia drugs; 4) synthesis and analysis of oligonucleotides; and 5) mechanistic studies of gold nanoparticle formation and reactivity.