CE-Uv-MS for bioorganic, medicinal, and materials chemistry

该申请要求资助与紫外可见和质谱检测器（CE-UV-MS）耦合的毛细管电泳系统。该技术将毛细管电泳（CE-UV）的短分析时间和极高分离效率与质谱检测器（MS）的分子量和结构信息相结合。该技术成功地分离和分析了带电化合物，如肽，氨基糖苷类抗生素，核酸和辅酶A衍生物。所申请的设备是本系4名PI研究项目所必需的。化学。许多其他专业人员也表示需要使用这一工具。CE-UV-MS增强或启用的研究项目包括：1）用于临床研究的植物提取物指纹图谱和活性成分鉴定; 2）P450酶的工程改造; 3）砷抗白血病药物的蛋白质靶点鉴定; 4）寡核苷酸的合成和分析;以及5）金纳米颗粒形成和反应性的机制研究。

This application requests funding for a capillary electrophoresis system coupled to Uv-Visible and mass spectrometry detectors (CE-Uv-MS). This technique combines the short analysis time and extraordinarily high separation efficiency of capillary electrophoresis (CE-Uv) with the molecular weight and structural information derived from the mass spectrometry detector (MS). The technique successfully separates and analyzes charged compounds such as peptides, aminoglycoside antibiotics, nucleic acids, and coenzyme A derivatives. The equipment applied for is essential to the research programs of 4 PIs in the Dept. of Chemistry. Many other PIs have expressed the need for access to this instrument. The research projects enhanced or enabled by the CE-Uv-MS, include: 1) fingerprinting of plant extracts for clinical studies and identification of the active ingredients; 2) engineering of P450 enzymes; 3) identification of protein targets of arsenical antileukemia drugs; 4) synthesis and analysis of oligonucleotides; and 5) mechanistic studies of gold nanoparticle formation and reactivity.