The role of tandem tudor domain proteins in RNAi

串联 tudor 结构域蛋白在 RNAi 中的作用

• 批准号: 341457-2007
• 负责人: Duchaîne, Thomas
• 金额: $ 2.05万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=458389
• 关键词: role; tandem; tudor; domain; proteins

RNA-mediated interference, or RNAi, is the specific shut down of a gene's expression by the exposure of cells to double-stranded RNA, or to its derivatives. This phenomenon has had a deep impact on biological sciences in the recent years, primarily for its use to experimentally examine the outcome of the loss of any gene's function in experiments. In the most recent years it is becoming clear that RNAi also serves numerous physiological purposes. Through a diverse variety of related processes, it can profoundly influence gene regulatory networks. In the current research proposal, we put forward an integrated strategy to address the molecular basis for one of these phenomena termed endogenous RNAi (or endoRNAi). EndoRNAi modifies gene expression by converging its silencing signals on chromatin (the physiological architechtures of DNA). We will examine the function of two related proteins required for endoRNAi, which encode tandem tudor domains. Our previous work supports a model wherein these proteins direct the RNAi machinery to reinforce the silenced state of genes. The current proposal will result in a better understanding of the biological functions of RNAi, of the underlying mechanisms, and further elucidate the functions of the tudor domains in gene regulation.

RNA介导的干扰，或RNAi，是通过将细胞暴露于双链RNA或其衍生物来特异性关闭基因的表达。近年来，这一现象对生物科学产生了深刻的影响，主要是因为它被用于实验研究任何基因功能丧失的结果。近年来，人们越来越清楚地认识到，RNAi还具有许多生理目的。通过各种各样的相关过程，它可以深刻地影响基因调控网络。在目前的研究计划中，我们提出了一个综合的策略来解决这些现象之一称为内源性RNAi（或endoRNAi）的分子基础。EndoRNAi通过将其沉默信号聚集在染色质（DNA的生理结构）上来改变基因表达。我们将研究endoRNAi所需的两个相关蛋白的功能，它们编码串联tudor结构域。我们以前的工作支持一种模型，其中这些蛋白质指导RNAi机制以加强基因的沉默状态。目前的建议将导致更好地了解RNAi的生物学功能，潜在的机制，并进一步阐明tudor结构域在基因调控中的功能。