Analysis of dynamic systems in cells using simultaneous 2-channel imaging

在其一生中，细胞复制其染色体，组织其细胞骨架以改变形状或移动，形成分离染色体的有丝分裂纺锤体，并最终分裂形成两个新的子细胞。我们对这些过程的一般特征知道得很多。每一个过程都由一系列步骤组成，有些步骤非常快——几分钟或几秒钟。检测和分析快速的步骤对科学家来说是一个巨大的挑战，并且限制了我们对过程如何完全准确或为什么失败的理解。了解每个步骤通常需要检测过程中每个步骤所需的两个或更多的工作分子。所要求的设备将使我们的显微镜能够同时捕捉两个或两个以上分子的活动或运动的快速变化。通过这种方式，我们对所有细胞共有的过程有了更深入的了解。

During its lifetime, a cell duplicates its chromosomes, organizes its cytoskeleton to change shape or to move, forms a mitotic spindle that segregates chromosomes, and ultimately cleaves to form two new daughter cells. We know a great deal about the general features of these processes. Each of these processes is composed of a series of steps, and some steps are completely very quickly- in a few minutes or seconds. The detection and analysis of the rapid steps is a huge challenge to scientists, and limits our understanding of how a process is completely accurately, or why it fails. Understanding each step frequently requires the detection of two or more of the working molecules needed for each step in the process. The equipment requested will allow our microscope to capture the rapid changes in activity or movement of two or more molecules at the same time. In this way we gain deeper understanding of the processes that are common to all cells.