UV/visible spectrophotometer

紫外/可见分光光度计

• 批准号: 359844-2008
• 负责人: Igamberdiev, Abir
• 金额: $ 1.26万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=365597
• 关键词: UV; visible; spectrophotometer

The requested Biochrom Ultrospec 4300 pro UV/Visible Spectrophotometer will be used for measurement of enzyme activities, estimation of metabolite concentrations in subcellular compartments of plant cells, determination of kinetic parameters of enzymes and calculation of metabolic fluxes in the cell. The instrument will be placed in the Laboratory of Plant Metabolism at Department of Biology, Memorial University of Newfoundland. Ultrospec 4300 pro satisfies high specifications for resolution, noise, stability, wavelength accuracy and reproducibility essential for fine research of plant metabolism and bioenergetics. It is a powerful tool for investigation of enzyme kinetics and calculation of metabolic fluxes based on the metabolic control theory. It will allow evaluating intensities of metabolic pathways in stress-induced processes in different settings. This piece of equipment is an integral component of most physiological and biochemical work aimed at understanding operation of bioenergetic organelles, such as mitochondria and peroxisomes. The availability of Biochrom Ultrospec 4300 will promote research on adaptation of trees and cereal crops to environmental stresses, including the hypoxic stress, drought, and pathogens. It will be also used for research of photosynthetic bacteria and plant-insect interactions.

所要求的Biochrom Ultrospecc 4300 PRO UV/Viour分光光度计将用于测量酶活性、估计植物细胞亚细胞室中的代谢物浓度、确定酶的动力学参数和计算细胞内的代谢通量。该仪器将放置在纽芬兰纪念大学生物系植物新陈代谢实验室。Ultrospecc 4300 PRO在分辨率、噪声、稳定性、波长精度和重复性方面满足了对植物新陈代谢和生物能量学的精细研究所必需的高规格。它是以代谢控制理论为基础的酶动力学研究和代谢通量计算的有力工具。它将允许在不同的环境下评估压力诱导过程中代谢途径的强度。这台设备是大多数生理和生化工作的组成部分，目的是了解生物能量器的运作，如线粒体和过氧化物酶。Biochrom Ultrospecc 4300的上市将促进树木和谷类作物对环境胁迫(包括低氧胁迫、干旱和病原体)的适应研究。它还将用于光合细菌和植物-昆虫相互作用的研究。