Genetic regulatory mechanisms: studies on genes responsive to ethanol

遗传调控机制：对乙醇敏感的基因研究

• 批准号: 6971-2008
• 负责人: Singh, Shiva
• 金额: $ 1.82万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=474190
• 关键词: Genetic; regulatory; mechanisms; studies; genes

The long-term goal of this research is to uncover and understand mechanisms underlying regulation of eukaryotic genes using mouse as the model and train graduates in the field of genetics. The genes for this research have been identified by mouse expression arrays using brain RNA from two strains; C57BL/6J and DBA/2J. The results have established that the strains differ for ~2% of the genes. Besides the strain specific (SS) differences we have identified genes that are ethanol responsive (ER) and strain specific ethanol responsive (SSER). This variation is critical in adaptation including sensitivity to external challenges and allowed us to propose a genomics approach to link this variation to sensitivities and behaviours (Singh et al 2007).This proposal deals with two questions;

本研究的长期目标是以小鼠为模型，揭示和理解真核生物基因调控的潜在机制，培养遗传学领域的研究生。这项研究的基因已经通过小鼠表达阵列使用来自两个品系的脑RNA进行了鉴定; C57 BL/6 J和DBA/2 J。结果已经确定，菌株在约2%的基因上存在差异。除了菌株特异性（SS）差异之外，我们还鉴定了乙醇响应性（ER）和菌株特异性乙醇响应性（SSER）的基因。这种变异在适应中是至关重要的，包括对外部挑战的敏感性，并使我们能够提出一种基因组学方法，将这种变异与敏感性和行为联系起来（Singh等人，2007年）。