Biochemical and imaging studies on type III secretion mechanisms

III型分泌机制的生化和影像学研究

• 批准号: 341435-2010
• 负责人: Coombes, BrianKenneth
• 金额: $ 4.08万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=482097
• 关键词: Biochemical; imaging; studies; type; III

Project Summary - The aim of this research is to understand the mechanisms of how bacteria use a specialized secretion apparatus called the type III secretion system as a way to colonize host animals. This secretion system injects dozens of bacterial proteins directly into host cells so that the bacteria can use these cells for their growth. Disabling the secretion system renders the bacteria unable to colonize their hosts and so understanding how this system works at a molecular level is important. We study type III secretion systems in bacteria that contaminate food and water supplies in Canada, as well as helpful bacteria that live in animals, in order to understand fundamental aspects of cell biology and bacterial ecology. Our focus has been on specialized bacterial proteins called chaperones that we believe help the type III secretion system function properly.

本研究的目的是了解细菌如何使用一种称为III型分泌系统的特殊分泌装置作为宿主动物定居的方式的机制。这种分泌系统将数十种细菌蛋白质直接注入宿主细胞，以便细菌可以利用这些细胞进行生长。禁用分泌系统会使细菌无法在宿主中定植，因此了解该系统在分子水平上的工作方式非常重要。我们研究污染加拿大食物和水源的细菌中的III型分泌系统，以及生活在动物体内的有益细菌，以了解细胞生物学和细菌生态学的基本方面。我们的重点一直是专门的细菌蛋白质称为伴侣，我们相信帮助III型分泌系统正常运作。