Genetic analysis of the sushi domain protein Ccdc80 ub zebrafish heart development

sushi结构域蛋白Ccdc80 ub斑马鱼心脏发育的遗传分析

• 批准号: 341545-2007
• 负责人: Scott, Ian
• 金额: $ 1.91万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=482150
• 关键词: Genetic; analysis; sushi; domain; protein

A fundamental question in heart biology is how this organ develops and assumes its mature, adult form. The heart must continue to maintain blood flow in the developing embryo while still growing and undergoing concerted cell movements that convert a linear heart tube into a complex structure. Due to its optical clarity, rapid external development, and amenability to genetic approaches, the zebrafish has become a powerful tool to study how genes regulate heart development. In a large-scale screen for genes that play important roles in heart development I have identified a mutation that affects how the endocardium (inner lining of the heart) and myocardium (muscular layer of the heart) communicate to ensure that the heart assumes proper shape and function. This mutation blocks production of a protein called Ccdc80. Of great interest is the fact that Ccdc80 is a novel, potentially secreted, protein of unknown function.

心脏生物学中的一个基本问题是这个器官如何发育并呈现其成熟的成人形式。心脏必须在发育中的胚胎中继续维持血流，同时仍然生长并经历协调的细胞运动，将线性心管转化为复杂的结构。由于其光学清晰度，快速的外部发育和对遗传方法的顺从性，斑马鱼已成为研究基因如何调节心脏发育的有力工具。在对心脏发育中起重要作用的基因的大规模筛选中，我发现了一种突变，它影响了内膜（心脏的内层）和心肌（心脏的肌肉层）如何沟通，以确保心脏具有正确的形状和功能。 这种突变阻止了一种名为Ccdc80的蛋白质的产生。Ccdc80是一种新的、潜在的分泌型蛋白质，功能未知，这一事实非常有趣。