Department Core Facility Plate Reader

部门核心设施车牌阅读器

• 批准号: 423270-2012
• 负责人: Lindinger, Michael
• 金额: $ 4.08万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495910
• 关键词: Department; Core; Facility; Plate; Reader

This request is for a state of the art plate reader that will serve the needs of 12 faculty researchers in the Department of Human Health and Nutritional Sciences, their 68 graduate students, and a similar number of undergraduate student projects annually. Five of these 12 faculty researchers are strongly supporting this application. The 12 faculty researchers either study various aspects of cellular metabolism, or need to measure cellular metabolites, enzyme activities or signalling agents to describe and quantify physiological or pathophysiological process in cells, tissues and organs. This new plate reader would replace an aging one (over 12 years old) that is increasingly unreliable, and reduce the load on another researcher's instrument that is heavily used. While there are two newer (less than 5 years old) plate readers in the Department, they 'belong' to researchers with large lab groups who needed dedicated equipment. The requested plate reader is therefore to provide a very good level of service to Departmental researchers needing to use a good plate reader with UV, luminescence, absorbance, fluorescence and kinetic capabilities.The equipment would also be used by post-doctoral fellows and graduate students working in the applicants' labs. This equipment is also important in the training of undergraduate students, of which we have increased numbers performing excellent, lab-based research projects. Learning how to use this equipment and processing samples for analysis comprises an important part of the training of these people.

这一要求是最先进的酶标仪，将服务于人类健康和营养科学系的12名研究人员的需求，他们的68名研究生，以及每年类似数量的本科生项目。这12名研究人员中有5名强烈支持这一应用。12名研究人员要么研究细胞代谢的各个方面，要么需要测量细胞代谢物，酶活性或信号传导剂，以描述和量化细胞，组织和器官中的生理或病理生理过程。这种新的酶标仪将取代日益不可靠的老化酶标仪（超过12年），并减少另一个研究人员大量使用的仪器的负荷。虽然有两个较新的（不到5岁）板读取器在该部门，他们“属于”的研究人员与大型实验室团体谁需要专用设备。因此，所要求的酶标仪将为需要使用具有紫外、发光、吸光度、荧光和动力学功能的酶标仪的部门研究人员提供非常好的服务水平。该设备也将供在申请人实验室工作的博士后研究员和研究生使用。这些设备在本科生的培训中也很重要，我们已经增加了执行优秀的实验室研究项目的人数。学习如何使用这些设备和处理样品进行分析是这些人员培训的重要组成部分。