Manipulating brain circuits using optogentic equipment to examine memory in mice

使用光设备操纵大脑回路来检查小鼠的记忆

• 批准号: 423336-2012
• 负责人: Josselyn, Sheena
• 金额: $ 8.25万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495919
• 关键词: Manipulating; brain; circuits; using; optogentic

Major scientific breakthroughs often stem from key technological advances. For example, the development of gene targeting techniques revolutionized biology by providing a set of tools that can be used to manipulate gene function within organisms. Recently, optogenetic techniques have been developed that provide a set of tools that can be used to manipulate neural function in behaving organisms with exquisite spatial and temporal control. This application is for the purchase of optogenetic equipment that will allow the joint applicants (Drs. Josselyn and Frankland) to modulate the activity of genetically distinct groups of neurons with pulses of light in behaving animals. This sort of technology is becoming widely used in our (and many other) research fields. Indeed, we have recently collaborated with Dr. Shoayu Ge at Stonybrook who has introduced us to this exciting optogenetic technology. We currently have a paper under review at Neuron detailing this experiment (Gu et al., under review). Both Dr. Josselyn and Dr. Frankland have exciting studies that require this equipment. However, we do not, at present, have access to this exciting technology in our own labs. The lack of the equipment, therefore, puts us at a disadvantage in a field in which this technology is becoming almost standard. With this equipment, we will be able to apply for additional operating funds and to conduct sophisticated important experiments that will give insights into how the brain encodes information.

重大的科学突破往往源于关键技术的进步。例如，基因靶向技术的发展通过提供一套可用于操纵生物体内基因功能的工具而彻底改变了生物学。 最近，已经开发了光遗传学技术，其提供了一套工具，可用于操纵具有精细空间和时间控制的行为生物体中的神经功能。 该申请用于购买光遗传学设备，该设备将允许联合申请人（Josselyn和Frankland博士）在行为动物中用光脉冲调节遗传上不同的神经元组的活动。 这种技术在我们（和许多其他）研究领域中得到了广泛的应用。 事实上，我们最近与Stonybrook的Shoayu Ge博士合作，他向我们介绍了这种令人兴奋的光遗传技术。 我们目前在Neuron上有一篇正在审查的论文，详细介绍了这个实验（Gu et al.，审查中）。 Josselyn博士和Frankland博士都有令人兴奋的研究需要这种设备。 然而，我们目前还无法在自己的实验室中获得这项令人兴奋的技术。 因此，缺乏设备使我们在这项技术几乎成为标准的领域处于不利地位。 有了这些设备，我们将能够申请额外的运营资金，并进行复杂的重要实验，从而深入了解大脑如何编码信息。