Fluorimetric analysis of voltage-gated ion channel gating

电压门控离子通道门控的荧光分析

• 批准号: 355660-2008
• 负责人: Claydon, Tom
• 金额: $ 2.19万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498050
• 关键词: Fluorimetric; analysis; voltage; gated; ion

Ion channels are proteins that form ion-selective pores within cell membranes. They control membrane excitability and are therefore critical determinants of cellular function. Voltage-gated potassium (Kv) channels, which are a very diverse family of ion channel, are highly selective for potassium ions (K+) and open (gates) in response to changes in the voltage across the cell membrane. Despite their critical role in regulating excitability in cardiac, nerve, and endocrine cells, dynamic structural information regarding key Kv channel gating mechanisms is lacking.

离子通道是在细胞膜内形成离子选择性孔的蛋白质。它们控制膜的兴奋性，因此是细胞功能的关键决定因素。电压门控钾（Kv）通道是一个非常多样化的离子通道家族，对钾离子（K+）具有高度选择性，并响应于跨细胞膜电压的变化而打开（门）。尽管它们在调节心脏、神经和内分泌细胞的兴奋性中起关键作用，但缺乏关于关键Kv通道门控机制的动态结构信息。