Carbohydrate Analysis Facility

点击翻译按钮获取中文摘要

Research in our laboratories concerns the structure and function relationship of carbohydrate-active enzymes, proteins that catalyse biochemical reactions involving sugars. Central to these investigations, involving bacterial, yeast, and plant physiology, is the need for the analysis of carbohydrates. To this end, the applicant used to maintain and operate a carbohydrate analysis facility for researchers at the University of Guelph and beyond for over 15 years. At the core of this facility was a Dionex high-pH anion-exchange chromatograph coupled with pulsed-amperometric detection for the resolution and detection carbohydrates at levels below one billionth of a gram. However, key elements of this system have failed and they need to be replaced. Given advances in technology, both in hardware and software, a completely new system is required. Thus, we propose to purchase a Dionex ICS-5000 Ion Chromatography System for Carbohydrate Analysis. This system utilising a CarboPac PA200 Analytical Column would be equipped with a pulsed electrochemical detector (PED) and gold electrode for detection of carbohydrates (and amino acids too) and an AS-AP Autosampler to manage the many samples requiring analysis for each experiment. The system is controlled by the Chromeleon 7 software.