Bioproducts analysis facilties

生物产品分析设施

• 批准号: 458813-2014
• 负责人: Clarke, Anthony
• 金额: $ 8.92万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=555414
• 关键词: Bioproducts; analysis; facilties

Research in our laboratories concerns the structure and function relationship of carbohydrate-active enzymes, proteins that catalyse biochemical reactions involving sugars. Central to these investigations, which focus on bacterial, yeast, and plant physiology and metabolism, is the need for the purification and analysis of biomolecules, such as proteins, carbohydrates, organic acids and alcohols. To this end, the principal applicant maintains and operates a number of liquid chromatography systems including carbohydrate analysis facility for researchers at the University of Guelph and beyond. At the core of this facility is a Dionex high-pH anion-exchange chromatograph coupled with pulsed-amperometric detection for the resolution and detection carbohydrates at levels below one billionth of a gram. However, samples have to been injected into the system manually thus compromising the efficiency of the service. To provide better service, we propose to purchase a Dionex AP-AS autosampler that interfaces to the existing equipment. Also, chromatography equipment used for the purification of the proteins and enzymes under investigation has deteriorated to point where it needs to be replaced. Hence, we also propose to purchase a dedicated protein purification system, an Akta Pure M1 chromatography system. Finally, we have a need to analyse for the concentration of alcohols in various biological samples by high-pressure liquid chromatography (HPLC). Alcohols do not absorb light and they are not ionic thus precluding the use of spectrophotometric or ion detectors that typically comprise HPLC systems. However, the detection of alcohols can be achieved by monitoring the change in refractive index that they cause in carrier solvents and so we propose to equip an existing HPLC system with a new RI detector.

我们实验室的研究涉及碳水化合物活性酶的结构和功能关系，催化涉及糖的生化反应的蛋白质。 这些研究的重点是细菌、酵母和植物的生理学和代谢，其核心是需要对生物分子进行纯化和分析，如蛋白质、碳水化合物、有机酸和醇。为此，主申请人维护并操作了许多液相色谱系统，包括用于圭尔夫大学及其他大学的研究人员的碳水化合物分析设施。 该设施的核心是一台Dionex高pH阴离子交换色谱仪，配有脉冲安培检测器，用于解析和检测十亿分之一克以下的碳水化合物。 为提供更佳服务，我们建议购买Dionex AP-AS自动进样器，以连接现有仪器。 此外，用于纯化研究中的蛋白质和酶的色谱设备已经恶化到需要更换的地步。 因此，我们还建议购买专用的蛋白质纯化系统，即Akta Pure M1色谱系统。 最后，我们需要通过高压液相色谱法（HPLC）分析各种生物样品中的醇类浓度。 醇不吸收光，并且它们不是离子的，因此排除了通常包括HPLC系统的分光光度或离子检测器的使用。 然而，醇类的检测可以通过监测它们在载体溶剂中引起的折射率变化来实现，因此我们建议为现有的HPLC系统配备新的RI检测器。