Modulation of nuclear T-tubules in endocardial endothelium by ET-1. Modulation des tubules-T nucléaires par l'ET-1 au niveau de l'endothelium endocardique

ET-1 对心内膜内皮细胞核 T 管的调节。

• 批准号: 203657-2010
• 负责人: Jacques, Danielle
• 金额: $ 1.97万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=505762
• 关键词: Modulation; nuclear; tubules; endocardial; endothelium

INTRODUCTION: Recently, intranuclear structures were identified in several cell lines such as Hela cells, human hepatoma SKHep1 cells, and mouse myoblast C2C12 cells. Very recently, our group identified, for the first time, similar intranuclear structures in normal human endocardial endothelial cells (EECs). These tubular-like invaginations were called by our laboratory nuclear T-tubules (NTTs) because of their resemblance to the plasma membrane T-tubules. We and others suggested that their presence may depend on the metabolism and activities of the cells such as proliferation. However, there are no information on whether these structures are effectively an extension of the nuclear envelope membranes and may possess, as the nuclear envelope membranes of human EECs, ET-1 receptors (ET-1Rs) and nuclear pores. Furthermore, there is no information on whether NTTs would affect ET-1 modulation of nuclear Ca2+ homeostasis and cell cycle of human endocardial endothelial cells. In addition, whether or not ET-1 modulates the presence or absence of NTTs and more particularly in human EECs, remains to be discovered.

前言：最近，在Hela细胞、人肝癌SKHep1细胞和小鼠成肌细胞C2C12等细胞系中发现了核内结构。最近，我们的团队首次在正常人的心内膜内皮细胞(EECs)中发现了类似的核内结构。这些管状凹陷被我们实验室称为核T管(NTT)，因为它们与质膜T管相似。我们和其他人认为，它们的存在可能取决于细胞的新陈代谢和活动，如增殖。然而，目前还没有关于这些结构是否是核包膜的有效延伸以及是否像人类EECS的核包膜一样具有ET-1受体(ET-1Rs)和核孔的信息。此外，关于NTTS是否会影响ET-1对人心内膜内皮细胞核钙稳态和细胞周期的调节，目前还没有相关信息。此外，ET-1是否调节NTT的存在或不存在，尤其是在人类EECs中，仍有待发现。