Fluorescent/UV/Vis spectrophotometry for the determination of biocompatibility, cell adhesion properties and quantitative analysis of different analytes

荧光/紫外/可见分光光度法用于测定不同分析物的生物相容性、细胞粘附特性和定量分析

• 批准号: 440210-2013
• 负责人: Tabrizian, Maryam
• 金额: $ 3.27万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=509714
• 关键词: Fluorescent; UV; Vis; spectrophotometry; determination

We are interested in developing biomimetic biomaterials as thin films, patterned surfaces or matrices for in vitro tissue engineering. In parallel, we investigate polyplexes/nanoplexes as carriers for (targeted) delivery of proteins, genetic materials and biologically active molecules to cells or tissues. Fluorescent and UV/Vis spectrophotometry are extremely sensitive analytical tools that are essential for conducting research in the aforementioned research activities. Coupled with fluorescence analysis, UV/Vis spectrophotometry allows for investigating and quantifying cell adhesion properties, cell viability, cell proliferation and differentiation in response to the new interfaces and substrates developed in our research activities. The particular feature of the requested spectrophotometer is its ability to measure absorbance in the UV range, as well as fluorescence /luminescence at a wide range of excitation/emission ranges, thus combining rapid and highly sensitive fluorescence and absorbance readings. This allows for further characterization of encapsulated or bioconjugated moieties and/or their release kinetics. This equipment is very complementary to the available HPLC, fluorescence microscopy and gel electrophoresis equipment in our laboratory, thus further facilitating reliable qualitative and quantitative analyses regarding biocompatibility of our novel biomaterials and interfaces. The proposed spectrophotometer system is also well suited for monitoring biomolecule release kinetic profiles in real time, and determining the encapsulation efficiency of the delivery systems investigated by our group. The requested system will replace the outdated 16-year old spectrophotometers that were rendered out of service since summer 2011. Currently, the group has very limited access to various other spectrophotometers found on campus; moreover, no access is available to either a UV/Vis or fluorescence spectrophotometry system that meets the required analytical needs. This greatly impedes our research progress and creates a detrimental situation for over 30 graduate students, fellows and research staff in our laboratories whom are heavily reliant on the frequent use of fluorescence and UV/Vis spectrophotometry for their projects.

我们致力于开发仿生生物材料，如薄膜，图案化表面或体外组织工程基质。与此同时，我们研究了聚合复合物/纳米复合物作为载体（靶向）递送蛋白质，遗传物质和生物活性分子到细胞或组织。荧光和紫外/维斯分光光度法是极其灵敏的分析工具，对于在上述研究活动中开展研究至关重要。结合荧光分析，UV/维斯分光光度法可用于研究和定量细胞粘附特性、细胞活力、细胞增殖和分化，以响应我们研究活动中开发的新界面和基质。所要求的分光光度计的特殊功能是其能够测量紫外范围内的吸光度，以及在宽范围的激发/发射范围内的荧光/发光，从而结合快速和高灵敏度的荧光和吸光度读数。这允许进一步表征包封的或生物缀合的部分和/或其释放动力学。该设备与我们实验室现有的HPLC、荧光显微镜和凝胶电泳设备非常互补，从而进一步促进了对我们新型生物材料和界面的生物相容性进行可靠的定性和定量分析。所提出的分光光度计系统也非常适合于监测生物分子释放动力学曲线在真实的时间，并确定我们的小组研究的递送系统的包封效率。所要求的系统将取代自2011年夏季以来停止使用的16年旧的过时的温度计。目前，该小组对校园内的各种其他光度计的使用非常有限;此外，无法使用满足所需分析需要的紫外/维斯或荧光分光光度计系统。这极大地阻碍了我们的研究进展，并为我们实验室的30多名研究生、研究员和研究人员创造了不利的局面，他们在项目中严重依赖于频繁使用荧光和紫外/维斯分光光度法。