Democratization of Mass Spectrometry Through Simplified Sample Preparation/Ionization for High Impact Applications

通过简化样品制备/电离实现高影响应用的质谱分析大众化

基本信息

  • 批准号:
    RGPIN-2022-03833
  • 负责人:
  • 金额:
    $ 5.61万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2022
  • 资助国家:
    加拿大
  • 起止时间:
    2022-01-01 至 2023-12-31
  • 项目状态:
    已结题

项目摘要

Chemical analysis forms the basis for discovery in a wide variety of disciplines (e.g. environmental, medical and "omics" related research areas). The development of analysis systems that are faster, require less sample, and offer increased sensitivity and compatibility with a variety of matrices are critical to probing e.g. biological and chemical systems. My research program proposal leverages our expertise in microfluidics, mass spectrometry, and separation science and will focus on novel methods to enhance, simplify and ruggedise both sample preparation and introduction for mass spectrometry, and apply them to high impact applications. The proposed program will involve four research thrusts. The first will focus on the use of hydrophobic/hydrophilic patterned surfaces that self-wet to quickly yield micro-droplet arrays to carry out rapid sample preparation (tissue imaging, liquid chromatographic archiving and preconcentration). The second will focus on enhancing a novel mass spectrometric ambient ionization interface (liquid micro-junction surface sampling probe, LMJ-SSP) to rapidly sample the broader chemical space of complex biological samples using a "gradient at the tip approach". Both thrusts one and two seek to further democratise mass spectrometry, taking it from specialised, well-trained operators to more routine field use in public health, and hospital settings.  The third thrust will utilise the LMJ SSP to characterise biofilms formed on different relevant surfaces. Biofilms protect the microbial community from external pressures, including antibiotics, immune cells and drinking water disinfection. Trainees will work in the Drinking Water Distribution Laboratory (DWDL) at Queen's and at the newly minted Centre for Health Innovation (Kingston General Hospital) to assess biofilm growth on relevant surfaces. Contrastingly, the microbes that make up biofilms produce a number of bioactive compounds for mutualistic benefit and/or as a survival tool. In collaboration with Dr. Avena Ross's group at Queen's will be using novel MS tools to examine different species of Psuedoalteromonas grown on different scaffolds to search for novel biosynthetic compounds. The fourth thrust  involves working with Dr. Natalie Agar, Harvard School of Medicine, USA (Cancer) and Dr. Sabeth Verpoorte, Groningen, The Netherlands (Liver) and the Molecular Navigation Group at Queen's to develop LMJ-SSP and) as a rapid tissue imaging tool for intra-operative assessment of tumour margins and liver function. Equity, Diversity, and Inclusion will continue to be a priority of my research program. HQP will be required to participate in formal EDI training programs organised through the University Human Rights and Equity Office. HQP will benefit from working at discipline interfaces where researchers will be trained on tissue preparation, chemical/biochemical safety, and state-of-the-art electrochemical, mass spectrometric, microbiological and microfabrication techniques.
化学分析构成了各种学科(例如环境,医学和“组学”相关研究领域)发现的基础。开发更快、需要更少样品并提供更高灵敏度和与各种基质的兼容性的分析系统对于探测例如生物和化学系统至关重要。我的研究计划提案利用了我们在微流体,质谱和分离科学方面的专业知识,并将重点放在新方法上,以增强,简化和强化样品制备和质谱引入,并将其应用于高影响力的应用。该计划将涉及四个研究重点。第一个将侧重于使用疏水/亲水图案化表面,自润湿,以快速产生微滴阵列进行快速样品制备(组织成像,液相色谱存档和预浓缩)。第二个将集中在增强一种新的质谱环境电离接口(液体微结表面采样探针,LMJ-SSP),以快速采样复杂的生物样品的更广泛的化学空间,使用“尖端方法梯度”。第一和第二个目标是进一步普及质谱技术,使其从专业的、训练有素的操作员应用到公共卫生和医院环境中的常规领域。第三个目标是利用LMJ SSP来检测不同相关表面上形成的生物膜。生物膜保护微生物群落免受外部压力,包括抗生素,免疫细胞和饮用水消毒。学员将在皇后学院的饮用水分配实验室(DWDL)和新成立的健康创新中心(金斯顿总医院)工作,以评估相关表面上的生物膜生长。相反,构成生物膜的微生物产生许多生物活性化合物,用于互利互惠和/或作为生存工具。与皇后学院的Avena Ross博士的研究小组合作,将使用新的MS工具来检查生长在不同支架上的不同种类的假交替单胞菌,以寻找新的生物合成化合物。第四个重点是与美国哈佛医学院的Natalie Agar博士(癌症)和荷兰格罗宁根的Sabeth Verpoorte博士(肝脏)以及皇后大学的分子导航小组合作,开发LMJ-SSP,并将其作为术中评估肿瘤边缘和肝功能的快速组织成像工具。公平,多样性和包容性将继续是我的研究计划的优先事项。HQP将被要求参加通过大学人权和公平办公室组织的正式EDI培训计划。HQP将受益于学科接口的工作,研究人员将接受组织制备,化学/生化安全以及最先进的电化学,质谱,微生物学和微加工技术的培训。

项目成果

期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
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Oleschuk, Richard其他文献

Organic-free, versatile sessile droplet microfluidic device for chemical separation using an aqueous two-phase system
  • DOI:
    10.1039/c8lc01121d
  • 发表时间:
    2019-02-21
  • 期刊:
  • 影响因子:
    6.1
  • 作者:
    Hermann, Matthias;Agrawal, Prashant;Oleschuk, Richard
  • 通讯作者:
    Oleschuk, Richard
Hydrophobic/hydrophilic patterned surfaces for directed evaporative preconcentration
  • DOI:
    10.1039/c9an01782h
  • 发表时间:
    2020-01-21
  • 期刊:
  • 影响因子:
    4.2
  • 作者:
    Tucker, Ben;Hermann, Matthias;Oleschuk, Richard
  • 通讯作者:
    Oleschuk, Richard
Photoinduced polymerization for entrapping of octadecylsilane microsphere columns for capillary electrochromatography
  • DOI:
    10.1021/ac061349t
  • 发表时间:
    2007-02-15
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    Xie, Ruixi;Oleschuk, Richard
  • 通讯作者:
    Oleschuk, Richard
Detection of Opioids on Mail/Packages Using Open Port Interface Mass Spectrometry (OPI-MS)

Oleschuk, Richard的其他文献

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{{ truncateString('Oleschuk, Richard', 18)}}的其他基金

Droplet-Based Analytical Chemistry Platforms Using Superhydrophobic and Superamphiphobic Surfaces
使用超疏水和超双疏表面的基于液滴的分析化学平台
  • 批准号:
    RGPIN-2016-04790
  • 财政年份:
    2021
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Discovery Grants Program - Individual
Droplet-Based Analytical Chemistry Platforms Using Superhydrophobic and Superamphiphobic Surfaces
使用超疏水和超双疏表面的基于液滴的分析化学平台
  • 批准号:
    RGPIN-2016-04790
  • 财政年份:
    2020
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Discovery Grants Program - Individual
Advanced sample introduction methods for mass spectrometry
用于质谱分析的先进进样方法
  • 批准号:
    521373-2018
  • 财政年份:
    2020
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Strategic Projects - Group
Droplet-Based Analytical Chemistry Platforms Using Superhydrophobic and Superamphiphobic Surfaces
使用超疏水和超双疏表面的基于液滴的分析化学平台
  • 批准号:
    RGPIN-2016-04790
  • 财政年份:
    2019
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Discovery Grants Program - Individual
Advanced sample introduction methods for mass spectrometry
用于质谱分析的先进进样方法
  • 批准号:
    521373-2018
  • 财政年份:
    2019
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Strategic Projects - Group
Droplet-Based Analytical Chemistry Platforms Using Superhydrophobic and Superamphiphobic Surfaces
使用超疏水和超双疏表面的基于液滴的分析化学平台
  • 批准号:
    RGPIN-2016-04790
  • 财政年份:
    2018
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Discovery Grants Program - Individual
Advanced sample introduction methods for mass spectrometry******
用于质谱分析的先进进样方法*****
  • 批准号:
    521373-2018
  • 财政年份:
    2018
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Strategic Projects - Group
Regional industry networking event with Queen's micro and nano focused researchers
与女王大学微米和纳米研究人员共同举办的区域行业网络活动
  • 批准号:
    508933-2017
  • 财政年份:
    2017
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Connect Grants Level 2
Droplet-Based Analytical Chemistry Platforms Using Superhydrophobic and Superamphiphobic Surfaces
使用超疏水和超双疏表面的基于液滴的分析化学平台
  • 批准号:
    RGPIN-2016-04790
  • 财政年份:
    2017
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Discovery Grants Program - Individual
Droplet-Based Analytical Chemistry Platforms Using Superhydrophobic and Superamphiphobic Surfaces
使用超疏水和超双疏表面的基于液滴的分析化学平台
  • 批准号:
    RGPIN-2016-04790
  • 财政年份:
    2016
  • 资助金额:
    $ 5.61万
  • 项目类别:
    Discovery Grants Program - Individual

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