Gas-phase chemistry of biological and metal-containing ions

生物离子和含金属离子的气相化学

• 批准号: 283305-2009
• 负责人: Siu, KWMichael
• 金额: $ 7.65万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=525666
• 关键词: Gas; phase; chemistry; biological; metal

Explosive and significant advances in proteomics have been made possible by innovative technological breakthroughs and conceptual developments in biological mass spectrometry (MS). Two key technological areas that are the foundations of protein identification in MS are ion generation and collision-induced dissociation (CID) of peptides. Our proposed research continues to aim at better understanding the fundamentals of these reactions and processes, as well as the structures and energetics of the resulting ions. This knowledge is important for eventually more accurate and efficient protein sequencing and identification, especially in applications to discover and identify disease biomarkers. In particular, our objectives are to explore the structures, energetics, formation, and fragmentation chemistries of (i) molecular radical cations of oligopeptides; these ions differ from the neutral peptides by having one fewer electron, have structures and properties that are different from their protonated counterparts, and provide unique information when subject to CID-induced gas-phase microsequencing; and (ii) metalated peptides and ternary complexes; metalation imparts unique and interesting structures and chemistries to the peptides. The proposed investigations will be carried out by means of a combination of experiments, including tandem MS, threshold CID measurements, infrared multiple photon dissociation (IRMPD) spectroscopy, and DFT calculations. Tandem MS experiments will be conducted on prototype and/or commercially available mass spectrometers, including triple-quadrupole, linear ion trap, and three-dimensional ion trap. Threshold CID measurements will be conducted on a triple-quadrupole mass spectrometer fitted with a ring ion guide as q2. All of the above hardware is available in the applicant's laboratory at York University. IRMPD spectroscopic experiments will be performed in the Free Electron Laser for Infrared Experiments (FELIX) facility at the FOM-Institute for Plasma Physics "Rijnhuizen" in Nieuwegein, the Netherlands.

生物质谱学的创新技术突破和概念发展使蛋白质组学的爆炸性和重大进展成为可能。MS中蛋白质识别的两个关键技术领域是离子产生和多肽的碰撞诱导解离(CID)。我们提议的研究继续致力于更好地理解这些反应和过程的基本原理，以及所产生的离子的结构和能量学。这一知识对于最终更准确和更有效的蛋白质测序和鉴定是重要的，特别是在发现和鉴定疾病生物标志物的应用中。特别是，我们的目标是探索(I)寡肽的分子自由基阳离子的结构、能量、形成和裂解化学；这些离子与中性多肽的不同之处在于少了一个电子，具有与质子化对应的离子不同的结构和性质，并在进行CID诱导的气相显微测序时提供独特的信息；以及(Ii)金属化肽和三元络合物；金属化赋予多肽独特而有趣的结构和化学。拟议的调查将通过一系列实验进行，包括串联质谱仪、阈值CID测量、红外多光子解离(IRMPD)光谱和密度泛函计算。串联质谱仪实验将在原型和/或商用质谱仪上进行，包括三重四极杆、线性离子陷阱和三维离子陷阱。阈值CID测量将在装有环离子导轨的三重四极质谱计Q2上进行。上述所有硬件都可以在申请人在约克大学的实验室获得。IRMPD光谱实验将在荷兰Nieuwegein的FOM等离子体物理研究所“Rijnhuizen”的自由电子激光红外实验(FELIX)设施中进行。