Alternative splicing in polyploids and hybrids

多倍体和杂种中的选择性剪接

• 批准号: 298228-2009
• 负责人: Adams, Keith
• 金额: $ 6.41万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=525926
• 关键词: Alternative; splicing; polyploids; hybrids

Polyploids, organisms with an extra set of chromosomes, are common among plants and several crop plants are polyploid. Polyploidy can lead to novel morphological and developmental characteristics, larger cell sizes, and new gene functions and expression patterns. The formation of polyploids often involves hybridization (crossing) between two species. The goal of this research is to understand the consequences of polyploidy and cross-species hybridization on gene expression with a focus on patterns of RNA splicing (removal of regions of the RNA during gene expression). I will assay the expression and RNA splicing of genes duplicated by polyploidy in Brassica napus, canola, including genes involved in specifying traits of agronomic importance such as seed oil synthesis and disease resistance. I will detect novel RNA splicing patterns that appear in plant hybrids during the first generation after a cross is made, using new technology to study RNA splicing patterns on a genome-wide scale in a model plant hybrid. The proposed research will provide the first insights into differences in RNA splicing patterns between duplicated genes in polyploid plants, and the first information on the prevalence of novel RNA splicing patterns that occur in hybrid plants and the types of genes that are affected. This research on RNA splicing in polyploids and hybrids is likely to have an impact on the fields of plant molecular biology, plant genomics, and molecular evolution. Some duplicate genes play a role in specifying traits of agronomic significance in plants, and some RNA splicing patterns in duplicated genes may be involved in conferring traits selected for during plant breeding. In particular, polyploid canola is important to Canada's economy and food supply and what is learned from studying RNA splicing in canola may lead to improvement of this crop plant. Understanding how RNA splicing patterns can change gene expression and function may lead to using RNA splicing as a tool for biotechnology applications to design crops with altered expression of genes of interest to create or improve desired characteristics.

多倍体是一种具有额外一组染色体的生物体，在植物中很常见，有几种作物是多倍体。多倍体可以导致新的形态和发育特征，更大的细胞尺寸，以及新的基因功能和表达模式。多倍体的形成通常涉及两个物种之间的杂交(杂交)。这项研究的目的是了解多倍体和跨物种杂交对基因表达的影响，重点是RNA剪接的模式(在基因表达过程中移除RNA区域)。我将分析甘蓝型油菜多倍体复制的基因的表达和RNA剪接，包括与种子油合成和抗病等重要农艺性状相关的基因。我将在杂交后的第一代检测植物杂交中出现的新的RNA剪接模式，使用新技术在模式植物杂交中研究全基因组范围的RNA剪接模式。这项拟议的研究将首次深入了解多倍体植物中复制基因之间的RNA剪接模式的差异，并首次提供关于杂交植物中出现的新的RNA剪接模式的流行度和受影响的基因类型的第一信息。多倍体和杂交种中RNA剪接的研究可能会对植物分子生物学、植物基因组学和分子进化等领域产生影响。一些重复基因对植物有农艺意义的性状起着决定作用，在植物育种中，重复基因中的一些RNA剪接模式可能参与了性状的选择。特别是，多倍体油菜籽对加拿大的经济和食品供应很重要，从油菜籽中的RNA剪接研究中获得的知识可能会导致这种作物的改良。了解RNA剪接模式如何改变基因的表达和功能，可能会导致将RNA剪接作为生物技术应用的工具，通过改变感兴趣基因的表达来设计作物，以创造或改善所需的特征。