Mechanical manipulation and characterization of biological cells - a micro/nanorobotics and microelectromechanical systems (MEMS) approach

生物细胞的机械操作和表征 - 微/纳米机器人和微机电系统 (MEMS) 方法

• 批准号: 312394-2010
• 负责人: Sun, Yu
• 金额: $ 3.5万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=526494
• 关键词: Mechanical; manipulation; characterization; biological; cells

Advances in biology and medicine require enabling techniques for mechanically manipulating cells and characterizing cellular properties at a high speed. The lack of these techniques is a barrier to areas such as genetic research, molecule screening, and disease diagnosis utilizing biophysical differences between normal and malignant cells. Compared with biochemical techniques, mechanical techniques for manipulating and characterizing biological cells are largely under explored. This research program will develop innovative tools, systems, and techniques for mechanically manipulating and characterizing single cells, pursuing three major strategic directions, namely: (1) high-speed microrobotic cell injection for molecule/gene screening; (2) nanorobotic manipulation of sub-cellular structures within scanning electron microsopes for cancer gene mapping; and (3) high-speed mechanical characterization of normal and malignant cells for disease diagnosis.

生物学和医学的进步需要能够机械操作细胞并高速表征细胞特性的技术。缺乏这些技术是遗传研究、分子筛选和利用正常细胞和恶性细胞之间的生物物理差异进行疾病诊断等领域的障碍。与生化技术相比，用于操纵和表征生物细胞的机械技术在很大程度上还处于探索之中。该研究计划将开发用于机械操作和表征单细胞的创新工具、系统和技术，追求三个主要战略方向，即：（1）用于分子/基因筛选的高速微型机器人细胞注射； (2) 在扫描电子显微镜内对亚细胞结构进行纳米机器人操作，以进行癌症基因图谱分析； (3)正常和恶性细胞的高速机械表征用于疾病诊断。