Exploring the evolution of molecular architecture of starch from granule initiation to maturity

探索淀粉分子结构从颗粒起始到成熟的演变

• 批准号: 355666-2013
• 负责人: Seetharaman, Koushik
• 金额: $ 2.11万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=527859
• 关键词: Exploring; evolution; molecular; architecture; starch

Starch remains a subject of intense scientific interest because of the structural complexity of this self-assembling macromolecule; the importance of starch in our diet; and its impact on health indicators. My long-term goal continues to be a focus on understanding starch granule architecture and relating the architectural makeup of starch to its functional behaviour in multi-component food systems and their consequent impact on human health. My short-term goal is to determine the molecular architecture of polymers at early stages of starch granule development to likely understand granule initiation, as well as to determine the structural differences between semi-crystalline and amorphous starch structures (i.e., alternating growth rings) within the starch granules. There is a paucity of research on transient starches, specifically on the characterization of amylopectin in terms of internal structure, composition of unit chains and molecular architecture. Furthermore, to the best of my knowledge only 3 papers have been published on the effect of diurnal rhythm on granule morphology, while none have studied the internal structure of the starch granules when grown under different light conditions. Therefore, the goals in this proposal are to 1) determine the molecular architecture of polymers at early stages of starch granule development, in both transient and storage starches, to likely understand granule initiation; and 2) understand the impact of diurnal propagation of granule on the molecular structure in amorphous versus semi-crystalline areas. Results from the proposed study will make significant contributions to a deeper understanding of starch granule internal structure and architecture - topics that are of great interest in the research community.

由于这种自组装大分子的结构复杂性，淀粉仍然是一个具有强烈科学兴趣的主题；淀粉在我们饮食中的重要性；以及它对健康指标的影响。我的长期目标仍然是专注于理解淀粉颗粒结构，并将淀粉的结构组成与其在多组分食物系统中的功能行为及其对人类健康的影响联系起来。我的短期目标是确定淀粉颗粒发育早期阶段聚合物的分子结构，以可能了解颗粒的形成，以及确定淀粉颗粒内半晶和非晶淀粉结构（即交替生长环）之间的结构差异。目前对瞬变淀粉，特别是支链淀粉在内部结构、单元链组成和分子结构等方面的研究还比较缺乏。此外，据我所知，关于昼夜节律对淀粉颗粒形态影响的论文只有3篇，而没有一篇论文研究了不同光照条件下淀粉颗粒的内部结构。因此，本提案的目标是：1)在淀粉颗粒发育的早期阶段确定聚合物的分子结构，在瞬态和储存淀粉中，以可能理解颗粒的形成；2)了解颗粒的昼夜传播对非晶区和半晶区分子结构的影响。该研究的结果将对深入了解淀粉颗粒的内部结构和结构做出重大贡献-这是研究界非常感兴趣的主题。