Hydrogel-based microfluidic cell culture system for controlled drug dosage effects

基于水凝胶的微流体细胞培养系统，用于控制药物剂量效应

• 批准号: 398613-2011
• 负责人: Cheung, Karen
• 金额: $ 4.29万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=529286
• 关键词: Hydrogel; based; microfluidic; cell; culture

OBJECTIVE: This project will develop a microfluidic system to culture, characterize, image, and screen cells within a single automated, portable platform, with large potential impact to improve cell-based drug screening. BACKGROUND: The 2-D monolayer-based method of cell culture is well established in molecular and cell biology. Recently, there has been increased interest in 3-D cell culture, in which cells are embedded in a material that mimics the ECM. Interaction between cells and the ECM in vivo regulates differentiation, migration, and many other functions. As a cancer model, the tissue culture environment can have a dramatic influence on cell behaviour, such as modified morphology, gene expression, and proliferation. This

目的：该项目将开发一种微流控系统，在一个自动化的便携式平台上培养、表征、成像和筛选细胞，对改善基于细胞的药物筛选具有巨大的潜在影响。背景：基于2-D单层细胞培养的方法在分子和细胞生物学中已经得到了很好的建立。最近，人们对3D细胞培养的兴趣越来越大，其中细胞被嵌入模拟ECM的材料中。细胞和ECM之间的相互作用在体内调节分化、迁移和许多其他功能。作为癌症模型，组织培养环境可以对细胞行为产生显著影响，例如改变形态、基因表达和增殖。这