Development of a non-linear optical laser scanning microscopy system for in situ examination of cartillage reconstructive procedures

开发用于软骨重建手术原位检查的非线性光学激光扫描显微镜系统

• 批准号: 428327-2011
• 负责人: Schweitzer, Mark
• 金额: $ 2.9万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=532530
• 关键词: Development; non; linear; optical; laser

Osteoarthritis of the knee is a health issue of increasing importance to Canada's ageing population. Recently, innovative cartilage repair techniques have been developed for the treatment of early to moderate stage osteoarthritis; however, there are few practical, non-invasive methods to evaluate the success of these techniques. Cartilage is made up primarily of the protein collagen populated by highly specialized cells known as chondrocytes. The number, organization and shape of these chondrocytes, acting in concert with the orientation of the collagen fibers determine the functional status of both native and repaired cartilage. Currently, biopsy, slicing, and staining are required to determine if a repair procedure has been successful. The obvious issue with this practice is the requirement for excision of a tissue sample, which could compromise structural integrity. Our intention is to apply non linear optical laser scanning microscopy (NLO-LSM) techniques to assess the integrity of cartilage in-vivo. We will take advantage of the strong NLO signals which occur naturally in cartilage illuminated by tightly focused pulsed-laser light. The NLO-LSM technique has been widely demonstrated to provide optical-microscopy-resolution images with very good contrast. The ability to use endogenous contrast for examination during surgery would reduce the need for cutting out a sample of the valuable implant tissue.

膝关节骨关节炎是一个健康问题，对加拿大的老龄人口越来越重要。最近，创新的软骨修复技术已被开发用于治疗早期至中期骨关节炎;然而，很少有实用的，非侵入性的方法来评估这些技术的成功。软骨主要由高度特化的细胞（称为软骨细胞）填充的蛋白质胶原组成。这些软骨细胞的数量、组织和形状与胶原纤维的取向一致，决定了天然和修复软骨的功能状态。目前，需要活检、切片和染色来确定修复手术是否成功。这种做法的明显问题是需要切除组织样本，这可能会损害结构完整性。我们的目的是应用非线性光学激光扫描显微镜（NLO-LSM）技术来评估体内软骨的完整性。我们将利用强NLO信号，它自然发生在软骨照射紧密聚焦脉冲激光。NLO-LSM技术已被广泛证明可以提供具有非常好对比度的光学显微镜分辨率图像。在手术过程中使用内源性造影剂进行检查的能力将减少切出有价值的植入物组织样本的需要。