Regulation of membrane fusion

膜融合的调节

• 批准号: 121595-2010
• 负责人: Trimble, William
• 金额: $ 1.97万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=545343
• 关键词: Regulation; membrane; fusion

Cells are bounded by a thin membrane that must be dynamically changed to permit cells to move, divide, or release bioactive signals to neighboring cells for brain function and hormonal control. My research program focuses on determining how cells control the fusion of small membranous packets called vesicles with the membranes within the cell. NSF is an enzyme responsible for controlling a critical step in fusion yet the mechanisms that control this enzyme are not understood. During the previous term of this grant we showed that NSF undergoes a modification which changes its activity. In addition, we have found that this modification occurs on different places in different cell types including immune cells and the brain. Our current research plan aims to determine what proteins cause this modification, why different sites are used in different cell types, and how this regulation affects the activity of NSF and the fusion of membranes. Due to the broad range of cellular functions controlled by NSF, the results of this work will be important to a wide range of biologists.

细胞以薄膜为界，薄膜必须动态改变，以允许细胞移动、分裂或向邻近细胞释放生物活性信号，以实现大脑功能和激素控制。 我的研究项目重点是确定细胞如何控制称为囊泡的小膜包与细胞内膜的融合。 NSF 是一种负责控制融合关键步骤的酶，但控制这种酶的机制尚不清楚。 在本次资助的前一个任期内，我们展示了 NSF 经历了改变其活动的修改。 此外，我们发现这种修饰发生在不同细胞类型的不同位置，包括免疫细胞和大脑。 我们目前的研究计划旨在确定哪些蛋白质导致这种修饰，为什么不同的细胞类型使用不同的位点，以及这种调节如何影响 NSF 的活性和膜的融合。 由于 NSF 控制的细胞功能范围广泛，这项工作的结果对于广大生物学家来说都很重要。