NSF-mediated disssembly of SNARE complexes

NSF 介导的 SNARE 复合体组装

• 批准号: RGPIN-2015-05202
• 负责人: Trimble, William
• 金额: $ 3.28万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629272
• 关键词: NSF; mediated; disssembly; SNARE; complexes

Precise membrane traffic is essential for cellular functions ranging from intracellular protein transport to targeted secretion and neurotransmitter release. The major aim of this research program is to understand how this regulation is achieved. Specifically, we are investigating the function of a pivotal component of the membrane fusion machinery, the ATPase N-ethylmaleimide sensitive factor (NSF). This regulation is extremely important in the brain as it controls neurotransmitter release. Membrane fusion is driven by the formation of coiled-coil complexes between SNARE proteins on vesicles and target membranes, and NSF is thought to regulate their assembly and disassembly. How NSF carries out these functions remains unclear although recent studies have suggested that NSF unwinds the SNAREs by untwisting them.

精确的膜运输对于从细胞内蛋白质运输到靶向分泌和神经递质释放的细胞功能至关重要。这项研究计划的主要目的是了解这种调节是如何实现的。具体来说，我们正在研究膜融合机制的一个关键组成部分，ATP酶N-乙基马来酰亚胺敏感因子（NSF）的功能。这种调节在大脑中非常重要，因为它控制神经递质的释放。膜融合是由囊泡上的SNARE蛋白和靶膜之间形成卷曲螺旋复合物驱动的，NSF被认为调节它们的组装和分解。NSF如何执行这些功能仍然不清楚，尽管最近的研究表明NSF通过解开它们来解开SNARE。