Bioengineering of liquid media for hypothermic storage of germplasm and enhancement of mammalian fertility

用于低温储存种质和增强哺乳动物生育能力的液体培养基生物工程

• 批准号: 462843-2014
• 负责人: Kan, Frederick
• 金额: $ 1.82万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=558095
• 关键词: Bioengineering; liquid; media; hypothermic; storage

Our aim is to develop new extenders that are compatible with fertility enhancing proteins to increase fertility in animals that are bred using artificial insemination. This study will be carried out in partnership with CryoStasis Ltd., a biotechnology company that focuses on development of novel media (solutions) for preservation of cells, tissues and organs. The company has invented new methodologies of preservation that avoid freezing and extend the lifespan of mammalian cells in a liquid phase by storing cells, tissues, and ultimately organs at sub-zero Celsius environments that are sufficiently low enough to suppress or arrest cellular metabolic activity. These inventions have led to commercialization of media (extenders) used in artificial insemination of farm animals for pork and dairy production. Recently, the company has identified the need for two new products:1) Expansion of the company's commercial product range beyond the current commodity-type of extenders to include high margin value-added storage solutions for laboratory animals, and 2) Inclusion in extenders of proteins that will increase fertility. Fertility has been decreasing in agriculturally important animals which are crucial sources of human food as a result of inbreeding and genetic selection for attributes other than fertility. At Queen's University we have recently discovered a novel glycoprotein (oviductin) that has the potential to enhance the fertilizing competence of mammalian sperm. In partnership with CryoStasis researchers we aim to develop new extenders that are compatible with fertility enhancing proteins to increase fertility in animals that are bred using artificial insemination. Given the problems with low fertility in farm animals new extenders

我们的目标是开发与生育增强蛋白兼容的新型扩展剂，以提高人工授精饲养动物的生育能力。这项研究将与CryoStasis有限公司合作进行，该公司是一家专注于开发用于保存细胞、组织和器官的新型介质（解决方案）的生物技术公司。该公司发明了新的保存方法，通过将细胞、组织和最终器官储存在零度以下的环境中，从而避免冷冻，延长哺乳动物细胞在液相中的寿命，这种环境低到足以抑制或阻止细胞代谢活动。这些发明导致了用于猪肉和乳制品生产的农场动物人工授精的媒介（扩展器）的商业化。最近，该公司已经确定了对两种新产品的需求：1)扩大公司的商业产品范围，超越目前的商品型扩展剂，包括用于实验动物的高利润增值存储解决方案；2)在扩展剂中包含可以提高生育能力的蛋白质。作为人类食物的重要来源，在农业上具有重要意义的动物中，由于近亲繁殖和对生育力以外的性状的遗传选择，其生育力一直在下降。在女王大学，我们最近发现了一种新的糖蛋白（输卵管蛋白），它有可能提高哺乳动物精子的受精能力。与CryoStasis研究人员合作，我们的目标是开发与生育增强蛋白兼容的新型扩展器，以提高人工授精饲养动物的生育能力。考虑到农场动物生育率低的问题，新的扩展器