Oligonucleotide attachment to three-dimensional porous membranes

寡核苷酸附着到三维多孔膜上

• 批准号: 469923-2014
• 负责人: Goh, MCynthia
• 金额: $ 1.78万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=563835
• 关键词: Oligonucleotide; attachment; three; dimensional; porous

ACGT Corporation (ACGT) has developed a market-ready DNA technology for use in medical diagnostics. Detection is achieved by capturing sample DNA in a flowing medium via attachment to beads placed along the flow path. However, the use of these beads is not ideal since extra steps for bead removal are required, but more importantly, the sensitivity of the assay is reduced. To solve these issues, ACGT would like to explore different substrates and attachment mechanisms to simplify the manufacturing process, improve sensitivity, and thereby reduce instrument cost.

ACGT公司（ACGT）开发了一种用于医疗诊断的市场化DNA技术。通过附着在沿着流动路径放置的珠子上，在流动介质中捕获样品DNA来实现检测。然而，使用这些珠子并不理想，因为需要额外的珠子去除步骤，更重要的是，降低了测定的灵敏度。为了解决这些问题，ACGT希望探索不同的基板和连接机制，以简化制造过程，提高灵敏度，从而降低仪器成本。