Tools for enhanced microbial synthesis of bioproducts.

用于增强生物产品微生物合成的工具。

• 批准号: 472550-2015
• 负责人: Levin, David
• 金额: $ 5.95万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564783
• 关键词: Tools; enhanced; microbial; synthesis; bioproducts.

A biotechnology and fermentation facility for bioengineering of biofuels and bioproducts was established in the Department of Biosystems Engineering in 2007. The availability of equipment in this facility enabled the applicants to secure significant levels of operating funds (> $1,000,000 per year from 2009 to 2014) and facilitated cutting edge research, which has resulted in 33 publications (mostly co-authored by Levin and Cicek et al.) in peer-reviewed journals. We wish to upgrade the biotechnology and fermentation facility to stream-line the scale-up production of bio-products (high-value lipids, anti-oxidants, and biopolymers) synthesized by microorganisms by expanding on our ability to monitor real-time fermentation reaction parameters such oxygen (O2) consumption and carbon dioxide (CO2) production by aerobic bacteria, biomass production (cell number increase), and changes in genome copy number and/or gene expression. We are currently developing batch, fed-batch, and continuous fermentation processes to optimize both cell mass production and yields of bioproducts, such as short chain length (scl-) and medium chain length (mcl-) polyhydroxylakanoate (PHA) polymers by bacteria (commonly referred to as bio-plastics), neutral lipids by oleaginous yeasts, and anti-oxidants by microalgae. These are aerobic processes in which the microorganisms consume O2 and release CO2 as a function of cell metabolism and growth. The ability to monitor and correlate instantaneous changes in cell mass with changes in O2 and CO2 concentrations will greatly facilitate the optimization of fermentation conditions for bioproduct synthesis. These tools will enable the optimization of growth of different microorganisms under different conditions in flask experiments before we scale-up the fermentation reactions in bioreactors. These tools will also enable us to quantify the growth of microorganisms at the level of genome copy number, as well as changes in levels of gene transcription in response to changes in growth conditions or in co-cultures containing two or more species. The requested tools will greatly enhance our ability to conduct leading edge research, and overcome barriers to biopolymer commercialization.

2007年在生物系统工程系建立了生物燃料和生物产品生物工程的生物技术和发酵设施。该设施的设备使申请人能够获得大量的运营资金（2009年至2014年每年超过100万美元），并促进了尖端研究，这导致了33篇出版物（主要由Levin和Cicek等人共同撰写）。发表在同行评议的期刊上我们希望升级生物技术和发酵设施，以简化生物产品的大规模生产通过扩大我们监测实时发酵反应参数的能力，例如好氧细菌的氧（O2）消耗和二氧化碳（CO2）产生，生物质生产，（细胞数量增加）和基因组拷贝数和/或基因表达的变化。我们目前正在开发分批、补料分批和连续发酵工艺，以优化细胞批量生产和生物产品的产率，例如通过细菌（通常称为生物塑料）的短链长度（scl-）和中链长度（mcl-）聚羟基链烷酸酯（PHA）聚合物，通过产油酵母的中性脂质，以及通过微藻的抗氧化剂。这些是好氧过程，其中微生物消耗O2并释放CO2作为细胞代谢和生长的函数。监测细胞质量的瞬时变化并将其与O2和CO2浓度的变化相关联的能力将极大地促进生物产品合成的发酵条件的优化。这些工具将能够在我们扩大生物反应器中的发酵反应之前，在烧瓶实验中在不同条件下优化不同微生物的生长。这些工具还将使我们能够在基因组拷贝数水平上量化微生物的生长，以及响应生长条件变化或含有两个或更多个物种的共培养物中基因转录水平的变化。所要求的工具将大大提高我们进行前沿研究的能力，并克服生物聚合物商业化的障碍。