Refrigerated incubator shaker and centrifuge for cell culture

用于细胞培养的冷藏培养箱摇床和离心机

• 批准号: 472729-2015
• 负责人: Suh, DaeYeon
• 金额: $ 1.6万
• 依托单位: University of Regina
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564826
• 关键词: Refrigerated; incubator; shaker; centrifuge; cell

Funds are requested for a refrigerated incubator-shaker and a refrigerated centrifuge, common equipment used for cell culture, cell harvesting and to overproduce and purify recombinant proteins. The refrigerated incubator will be used to culture bacterial cells and to overproduce recombinant protein at low temperature, which can lead to higher yields of correctly folded, soluble protein for characterization. The refrigerated centrifuge, used to harvest cells and to separate soluble proteins from insoluble debris in cell homogenates, will replace centrifuge that is no longer functional on a regular basis. Such equipment is among the most widely used in modern biochemistry laboratories, and is not only a vital part of our research but also an integral component for training highly qualified personnel. The equipment will be used to study the evolution of biosynthetic pathways for plant extracellular matrices (Suh) and characterize the response of microbes to external stimuli (Dahms). The knowledge generated from Suh's research should prove useful in manipulating unnatural biopolymers, possibly leading to novel applications in agriculture (crop/forest control), biotechhnology (biodegradable polymer) and medicine (drug carrier). Dahms' research has considerable potential to yield benefits to the environment and human health by determining the physiological effects to cells in response to persistent low-level pesticide exposure, antifungal drugs and metals. Studying the effects on human cells, pathogens and pathogen-host interactions will ultimately shed light on microbial virulence, disease susceptibility and progression.

要求提供资金，用于购置一个冷冻培养箱-振荡器和一个冷冻离心机，这些是用于细胞培养、细胞收获以及过量生产和纯化重组蛋白质的通用设备。冷冻培养箱将用于培养细菌细胞，并在低温下过量生产重组蛋白，这可以导致更高产量的正确折叠的可溶性蛋白用于表征。冷冻离心机用于收获细胞和分离细胞匀浆中的可溶性蛋白质和不溶性碎片，将取代不再正常工作的离心机。这些设备是现代生物化学实验室中使用最广泛的设备之一，不仅是我们研究的重要组成部分，也是培养高素质人才的重要组成部分。该设备将用于研究植物细胞外基质（Suh）生物合成途径的演变，并表征微生物对外部刺激（Dahms）的反应。从Suh的研究中产生的知识应该证明在操纵非天然生物聚合物方面是有用的，可能会导致农业（作物/森林控制），生物技术（可生物降解聚合物）和医学（药物载体）的新应用。Dahms的研究具有相当大的潜力，通过确定细胞对持续低水平农药暴露、抗真菌药物和金属的生理影响，对环境和人类健康产生益处。研究对人类细胞、病原体和病原体-宿主相互作用的影响将最终揭示微生物的毒力、疾病易感性和进展。