A cell culture incubator-shaker system for environmental biotechnology research

用于环境生物技术研究的细胞培养培养箱摇床系统

• 批准号: 359215-2008
• 负责人: Ghoshal, Subhasis
• 金额: $ 2.91万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=361293
• 关键词: cell; culture; incubator; shaker; system

An advanced incubator-shaker system that provides a range of temperature and atmospheric conditions for growing microbial cultures in a laboratory is being requested.  The incubator-shaker system will be used to grow microorganisms that are important for biodegradation of potentially toxic hydrocarbon compounds often found at contaminated sites as well as microorganisms that are required for efficient biological treatment of municipal wastewater.  Thus this instrument will support research in vital areas of environmental biotechnology.The incubator-shaker system will be used to support collaborative and individual research of two applicants.  A major research program for which the instrument will be used is aimed at developing strategies for rapid clean-up of petroleum-contaminated soils, particularly in cold climates. The incubator-shaker system will allow us to grow various pollutant-degrading bacteria that have strong potential for degrading spilled oil and solvents at contaminated industrial sites.  The microorganisms grown will be evaluated for their pollutant degradation potential and for their surface characteristics.  The information will be used to determine how pollutant-degrading bacteria can be effectively delivered through groundwater to impacted areas of contaminated sites, and to determine the environmental conditions which are required to allow pollutant-degrading microorganisms at contaminated sites to thrive, especially in cold regions.The incubator-shaker will also be used for studies aimed at improving the performance of biological wastewater treatment processes. A number of microorganisms found in municipal wastewater will be studied to characterize the complex microbial population dynamics, genomics, and to perform metabolic modelling to obtain a comprehensive description of the biological processes in various treatment systems.

需要一种先进的培养箱-振荡器系统，为实验室中的微生物培养提供一系列温度和大气条件。振荡器系统将用于培养微生物，所述微生物对于通常在污染场所发现的潜在有毒烃化合物的生物降解是重要的，以及微生物，所述微生物对于城市废水的有效生物处理是必需的。将支持环境生物技术重要领域的研究。培养箱-振荡器系统将用于支持两个申请人的合作和个人研究。该仪器将用于一个主要研究计划，旨在制定快速清理石油污染土壤的策略，特别是在寒冷气候下。培养-振荡系统将使我们能够培养各种污染物降解细菌，这些细菌对污染工业场地的溢油和溶剂具有很强的降解潜力。将对培养的微生物的污染物降解潜力和表面特征进行评估。这些信息将用于确定污染物-降解细菌可以通过地下水有效地传递到受污染场地的受影响区域，并确定使污染物降解微生物在受污染地点茁壮成长所需的环境条件，特别是在寒冷地区。培养-振荡器还将用于旨在提高生物废水处理工艺性能的研究。将研究城市废水中发现的一些微生物，以表征复杂的微生物种群动态，基因组学，并进行代谢建模，以获得各种处理系统中生物过程的全面描述。