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A cell culture incubator-shaker system for environmental biotechnology research

A cell culture incubator-shaker system for environmental biotechnology research
用于环境生物技术研究的细胞培养培养箱摇床系统
批准号:
359215-2008
负责人:
Ghoshal, Subhasis
金额:
$2.91万
依托单位:
依托单位国家:
加拿大
项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
财政年份:
2007
资助国家:
加拿大
项目状态:
已结题
起止时间:
2007-01-01 至 2008-12-31

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中文摘要
翻译
需要一种先进的培养箱-振荡器系统,为实验室中的微生物培养提供一系列温度和大气条件。 培养箱-振荡器系统将用于培养微生物,这些微生物对污染场地经常发现的具有潜在毒性的碳氢化合物的生物降解至关重要,还将用于培养有效生物处理城市废水所需的微生物。 因此,该仪器将支持环境生物技术重要领域的研究。培养箱-振荡器系统将用于支持两个申请人的合作和个人研究。 将使用该仪器的一个主要研究方案旨在制定快速清理石油污染土壤的战略,特别是在寒冷气候下。培养箱-振荡器系统将使我们能够培养各种污染物降解细菌,这些细菌具有降解受污染工业场所的溢油和溶剂的强大潜力。 将对培养的微生物的污染物降解潜力和表面特性进行评价。 这些信息将用于确定污染物降解细菌如何通过地下水有效地传递到受污染场地的受影响区域,并确定污染场地的污染物降解微生物茁壮成长所需的环境条件,特别是在寒冷地区。培养箱-振荡器还将用于旨在改善生物废水处理过程性能的研究。将研究城市废水中发现的一些微生物,以表征复杂的微生物种群动态,基因组学,并进行代谢建模,以获得各种处理系统中生物过程的全面描述。
英文摘要
An advanced incubator-shaker system that provides a range of temperature and atmospheric conditions for growing microbial cultures in a laboratory is being requested.  The incubator-shaker system will be used to grow microorganisms that are important for biodegradation of potentially toxic hydrocarbon compounds often found at contaminated sites as well as microorganisms that are required for efficient biological treatment of municipal wastewater.  Thus this instrument will support research in vital areas of environmental biotechnology.The incubator-shaker system will be used to support collaborative and individual research of two applicants.  A major research program for which the instrument will be used is aimed at developing strategies for rapid clean-up of petroleum-contaminated soils, particularly in cold climates. The incubator-shaker system will allow us to grow various pollutant-degrading bacteria that have strong potential for degrading spilled oil and solvents at contaminated industrial sites.  The microorganisms grown will be evaluated for their pollutant degradation potential and for their surface characteristics.  The information will be used to determine how pollutant-degrading bacteria can be effectively delivered through groundwater to impacted areas of contaminated sites, and to determine the environmental conditions which are required to allow pollutant-degrading microorganisms at contaminated sites to thrive, especially in cold regions.The incubator-shaker will also be used for studies aimed at improving the performance of biological wastewater treatment processes. A number of microorganisms found in municipal wastewater will be studied to characterize the complex microbial population dynamics, genomics, and to perform metabolic modelling to obtain a comprehensive description of the biological processes in various treatment systems.
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